Tsis tshua muaj siab chromataphy tag
2.Chromatogratographic Kem (Hloov Hom)
3.Chromatographic Tag (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
Tsis tshua muaj siab chromataphy tagyog cov tseem ceeb hauv cov kua hauv cov kua chromatography thev naus laus zis, siv dav hauv Biochemaceuticals, biophechnology, thiab lwm cov teb uas xav tau kev sib cais thiab cov tshuaj sib cais. As an important component of liquid chromatography technology, it plays a significant role in fields such as biochemistry, biopharmaceuticals, and biotechnology. Los ntawm kev xaiv cov ntsaws ruaj ruaj, ua kom zoo sib cais, thiab kev ua haujlwm zoo thiab kev sib cais ua tau zoo thiab ua kom huv ntawm ntau hom kev sib xyaw tuaj yeem ua tiav. Nrog rau kev txhim kho cov fillers tshiab, daim ntawv thov ntawm cov thev naus laus zis ntse, thiab kev ua haujlwm tau zoo thiab cov ntawv thov kev ua tau zoo thiab cov khoom siv tsis zoo thiab ua txuas ntxiv nthuav dav thiab txhim kho.
Chaw ua uas tsis tau haus
Cov ntsiab cai yooj yim
Cov Chromatographic kem yog cov tseem ceeb tivthaiv ntawm chromatographic thev naus laus zis, thiab nws cov cai ua haujlwm tau yog nyob ntawm cov khoom siv tshuaj uas yog cov khoom siv thiab cov khoom siv Chromatographic Thaum cov qauv hla dhau ntawm cov chromatographic carun, lub sij hawm ntawm cov sib txawv ntawm cov sij hawm sib txawv, ua rau muaj cov sijhawm nyob hauv colun, yog li ua tiav kev sib cais. Qhov txheej txheem sib cais no yog ua tiav los ntawm kev faib cov qauv ntawm cov qauv ntawm cov qauv mobile (roj lossis ua kua) thiab theem chaw nres tsheb hauv qab cov cab kuj. Nyob rau hauv qis-siab chromatography, lub xov tooj mobile feem ntau dhau los ntawm cov zaub mov chomatography ntawm lub siab qis, ua rau cov txheej txheem sib cais, ua kom cov txheej txheem sib cais thiab cov tswj tau ntau dua.
Cov qauv thiab hom
Tsis tshua muaj siab chromataphy tagFeem ntau muaj li ntawm peb ntu: Kem Tube, Kem lub taub hau, thiab ntim. Lub colun raj yog qhov tseem ceeb ntawm cov chromatographic colun, feem ntau yog ua los ntawm iav lossis cov khoom siv polymer, nrog ib qho ntev ntawm 2440 hli. Lub taub hau colun nyob ntawm ob qho kawg ntawm kem raj thiab raug siv los txuas cov khoom sib txuas xws li infusion twj, txhaj li qub, thiab detectors. Cov khoom ntim yog qhov tseem ceeb ntawm cov chromatographic colun, uas txiav txim qhov sib cais ua haujlwm thiab kev siv tau ntawm kem.
Raws li lawv cov kev siv sib txawv, qis-siab chromatography txhua tus tuaj yeem faib ua kev ntsuas cov kab mob duromatogrphy thiab npaj cov kab mob chromatogrophy. Kev tshawb xyuas cov chromatogrphy coluns feem ntau yog siv rau cov qauv tshuaj xyuas thiab kev tshuaj xyuas, nrog cov khoom muaj peev xwm loj thiab sib cais me me, tab sis nrog kev ua kom muaj peev xwm me me. Kev npaj cov kua roj ntsha tau siv rau cov qauv npaj thiab ua kom muaj peev xwm loj, tab sis colun ua haujlwm muaj txiaj ntsig thiab kev sib cais kom tsawg me ntsis rau kev tawm tsam cov chromatoGrphy kem.
Cov khoom ntim
Ntim yog qhov tseem ceeb ntawm cov khoom siv ua kom zoo, uas txiav txim siab kev sib cais ua haujlwm thiab kev siv tau ntawm kem. Cov ntim ntawm qis-siab cov chromatogrphy coluns feem ntau yog ua rau cov khoom muag muag xws li, nrog cov ntaub ntawv hluav taws xob, muaj qee cov ntaub ntawv me me ntawm 40130 μ m ). Cov neeg ua haujlwm no muaj cov khoom siv lub cev sib txawv thiab tshuaj lom neeg, xws li polarity, hydrophobicity, nqi, thiab tuaj yeem cuam tshuam nrog cov qauv molecules nyob rau ntau txoj kev kom ua tiav kev sib cais.
Cov kev xaiv ntawm cov fillers yog qhov tseem ceeb rau kev sib cais ntawm cov khoom lag luam qis suromatogrphy coluns. Cov tub lim sib txawv yog tsim rau ntau hom kev kuaj thiab sib cais kom ua. Piv txwv li, ntuj polysaccharide fiber xws li pectin thiab agarose yog qhov haum rau kev sib cais thiab huv ntawm biomolecules; Cellulose raws cov filters yog tsim nyog rau kev sib cais ntawm cov organic me me molecule compounds; Cov khoom siv hluavtaws polymer muaj cov ntau ntawm cov kev siv cuam tshuam thiab tuaj yeem siv los cais ntau hom sib txawv.
Muab piv
Kom tau txais kev nkag siab ntau dua ntawm cov yam ntxwv thiab cov txiaj ntsig ntawm cov khoom lag luam chromatogrphy qis, peb tuaj yeem sib piv ntawm cov roj ntsha siab cov depomatogrophy kem.
(1) Kev khiav hauj lwm siab:
Cov kev ua haujlwm siab ntawm cov khoom lag luam qis cov nplai yog qhov tsawg, feem ntau nyob rau hauv ntau ntawm 1 {2000} 1000 psi (0.770 bar); Cov kev ua haujlwm siab ntawm cov tshuaj siab cov kua chromatoggrophy yog qhov tseem ceeb, feem ntau nqa tawm ntawm kev ntsuas siab tshaj 2000 psi (140 bar). Qhov no ua rau cov kua nplaum uas muaj zog qis dua ntais ntau ruaj khov thiab tswj tau lub sijhawm ua haujlwm, txo qis kev pheej hmoo ntawm cov ntsuas kev puas tsuaj.
(2) Kev sib cais txuag:
Txawm hais tias siab-siab kua kua duromatogrphy Colums muaj kev sib cais siab dua muaj cov txiaj ntsig tshwj xeeb hauv kev sib cais loj molecule sib txuas thiab cov khoom ntuj. Ib qho ntxiv, qis-siab chromatography txhua tus tau txais txiaj ntsig zoo dua, thiab hloov tau yooj yim, ua rau lawv muaj kev lag luam xws li kev kuaj thiab khoom lag luam biological biological.
(3) Cov Kev Tsim Kho Kev Tsim Kho:
Kev kub siab kua cov kua txwv yuav tsum tau nruab nrog cov khoom siv kub siab xws li cov kev lag luam siab los ua kom lawv cov haujlwm ib txwm; Nws muaj qis dua yuav tsum tau ua rau kev teeb tsa kev teeb tsa thiab tuaj yeem tsav los ntawm cov khoom siv xws li cov twj tso kua mis. Qhov no ua rau cov kua muag qis cov tawv txwv cov roj ntsha ntau dua tus nqi ntawm cov ntsiab lus ua tau zoo thiab txij nkawm.
(4) daim ntawv thov tau:
Tsis tshua muaj siab chromataphy tagyog tsim nyog rau kev sib cais thiab ua kom huv cov khoom lag luam, cov protein, nucleic acids, nrog rau cov tsiaj txhu me, nrog rau cov organic me me molecule sib txuas; Kev kub siab kua cov kua txwv cov roj duromatogrophy yog qhov tsim nyog sib cais thiab sib cais ntawm cov kab txiav, xws li tshuaj xyuas tshuaj yeeb thiab saib xyuas ib puag ncig.
Kev ua haujlwm thiab txij nkawm
Kev ua haujlwm tau zoo thiab kev saib xyuas yog qhov tseem ceeb rau kev tswj hwm kev ua tau zoo thiab nthuav tawm cov kev pabcuam lub neej ntawm cov khoom lag luam qis cov tawv nqaij tawv. Nov yog qee qhov tseem ceeb ua haujlwm thiab cov lus qhia txij nkawm txij nkawm:
Npaj ua ntej ua haujlwm:
Xyuas kom cov kev sib txuas yog ntawm cov chromatography thiab cov kua chromatography.
Kuaj seb puas muaj pes tsawg leeg thiab cov nqi ntawm cov theem mobile ua tau raws li qhov yuav tsum tau ua.
Yaug cov chomatography sab nrog ib qho tsim nyog hnyav kom tshem cov seem.
Ua piv txwv:
Nco ntsoov tias tus qauv tau dhau los ua ntej tsim nyog, xws li pom, centrifugation, thiab lwm yam, ua ntej txhaj tshuaj kom tshem tawm impurities thiab hais.
Tswj cov kev txhaj tshuaj ntim thiab concentration ntawm cov qauv kom tsis txhob overloading uas yuav ua rau muaj kev txo qis hauv kab ntawv.
Txawb theem tswj:
Saib xyuas tus nqi ntws thiab lub siab ntawm cov theem mobile kom ntseeg tau tias lawv nyob hauv cov ntau yam.
Nquag xyuas cov muaj pes tsawg zaus thiab muaj kev ruaj khov ntawm theem mobile kom tsis txhob muaj paug thiab tsis zoo.
Tswj kub:
Tswj qhov kub ntawm cov chromatographic kem thiab cov theem mobile los txo qhov cuam tshuam ntawm thermal cuam tshuam ntawm kev sib cais.
Ua kom muaj kev ruaj ntseg thiab kev ntseeg tau ntawm qhov ntsuas kub kom tsis txhob sib cais tsis ruaj khov los ntawm kev kub ntxhov.
Kev ntxuav thiab tsim dua tshiab:
Nquag ntxuav cov vosporaphy Partratography sab nrog cov kuab tshuaj uas tsim nyog los tshem cov chaw nyob thiab cov paug.
Rau cov chromatogramy colums uas tsis tau siv rau lub sijhawm ntev, ua kev txhim kho kev txhim kho kom rov qab ua lawv cov kev ua tau zoo.
Cia thiab Txij Nkawm:
Thaum tsis siv, sau cov khawb ntoo chomatography nrog ib cov kuab tshuaj uas tsim nyog thiab khaws nws ntawm qhov ntsuas kub kom tsim nyog.
Nquag kuaj xyuas cov xwm txheej thiab kev ua tau zoo ntawm cov khawb ntoo chomatography, thiab hloov cov khoom siv puas lossis muaj hnub nyoog lossis cov khoom siv laus.
Cov Ntaubntawv thiab Kev Ntsuam Xyuas:
Sau cov kev ua haujlwm, sib cais efficiency, thiab chromatographic kem rau txhua qhov kev sim.
Txheeb xyuas cov ntaub ntawv sim, ntsuas kev hloov pauv hauv kev ua tau zoo ntawm chromatographic txhua, thiab siv ntsuas kom haum thiab ua kom zoo dua.
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov hauv qab no yog qee kis kev tshawb fawb ntawm kev siv cov roj nplaum chomatography cov txiaj ntsig zoo ntawm cov khoom siv chomatography muaj ntau yam:
(1) Kev sib cais protein:
Cais protein sib xyaw ua ke siv ion txauv cov tshuaj pleev ib ce. Los ntawm kev kho cov ph thiab ionic lub zog ntawm cov theem mobile, xaiv sib cais ntawm cov protein sib txawv tau ua tiav.
Qhov hnyav molecular ntawm cov protein tau kuaj los ntawm gel filtration enomatography sab. Cov protein sib txawv tau sib cais thiab ua kom huv raws li lawv qhov hnyav molecular.
(2) nucleic acid rho tawm:
Siv AGINATE CHOMATORYIESBIOR cim rau tshwj xeeb sib cais ntawm nucleic acids. Los ntawm kev qhia txog cov lus qhia txog ligand, xaiv los khi thiab sib cais ntawm cov kua qaub tshwj xeeb yog ua tiav.
Lub nucleic acid sib tov yog ntxiv kom huv los ntawm ion pauv hloov lossis gel filtration kem kom tshem tawm impurities thiab kuab paug.
(3) sib cais ntawm cov qauv me me magecule:
Cais cov me me molecule compounds siv ib theem sab nraud quav. Los ntawm kev kho cov muaj pes tsawg leeg thiab polarity ntawm mobile theem, kev sib cais ntawm cov molecule me me nrog cov polarities sib txawv.
Kev xaiv sib cais ntawm cov molecule me me nrog cov pab pawg tshwj xeeb uas siv cov theem ua kom tiav chomatographized sab.
(4) Kev sib cais tej khoom ntuj:
Kev sib cais thiab kev ua kom huv ntawm cov khoom xyaw ua haujlwm hauv cov nroj tsuag cov tshuaj siv siv cov roj qis cov denomatorahy. Los ntawm kev ua kom haum kev hloov kho thiab xaiv cov fillers uas tsim nyog, biologically dhia sib txuas tau tau ua tiav.
Kev siv ntawm cov roj nplaum uas tsis tshua muaj zog chomatography rau kev sib cais thiab kev txheeb xyuas cov metabolites hauv microbial fermentation broth muab cov neeg sib tw rau kev txhim kho tshuaj tshiab.
Cov kev tshawb fawb no tau pom tias muaj kev siv thoob plaws thiab kev ua tau zoo ntawmTsis tshua muaj siab chromataphy tagnyob rau ntau daim teb. Los ntawm kev xaiv cov chratterographic txhua thiab kev sib cais ua tau zoo thiab ua kom huv ntawm ntau hom kev sib txuas tuaj yeem ua tiav.
Cim npe nrov: Tsawg lub siab chromataphy tag, Tuam Tshoj tsis tshua muaj siab chromataphy Txhua cov tuam ntxhab, cov chaw muag khoom, Hoobkas
Tom ntej
Protein ib chromatography SabXa kev nug
