Kem chromatography khoom
2.Chromatogratographic Kem (Hloov Hom)
3.Chromatographic Tag (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
Kem chromatography khoom, Nws yog qhov sib cais feem ntau thiab cov qauv ua kom huv hauv kev siv computer tom nyiag. Nws siv cov qauv adsorption sib txawv ntawm txhua qhov sib xyaw ua ke rau theem sib xyaw, thiab cais cov tshuaj ntawm theem kev npaj txhij. Nws yog qhov tseem ceeb rau kev ua CC kev sim, thiab nkag siab tias nws cov qauv thiab kev ua haujlwm yog qhov tseem ceeb rau kev ua haujlwm kom ua haujlwm tau. Raws li ib qho ntawm cov cuab yeej tseem ceeb ntawm lub computer tomography, nws muaj ntau cov kev siv hauv kev ua liaj ua teb xws li Chemistry, thiab kev tshawb fawb ib puag ncig, thiab kev tshawb fawb ib puag ncig. To taub nws cov txheej txheem theem pib, hom kev ua haujlwm, thiab cov teb ua haujlwm yog qhov tseem ceeb ntawm kev sim ua haujlwm kom raug thiab tau txais kev sim ua kom raug. Nrog rau kev nce qib txuas ntxiv ntawm kev tshawb fawb thiab thev naus laus zis thiab kev nthuav dav ntawm daim ntawv thov kev ua haujlwm, nws yog kev txhawb nqa ib puag ncig rau kev tshawb fawb thiab kev tsim khoom.
Chaw ua uas tsis tau haus
Yooj yim sib xyaw
Kem chromatography khoomMas muaj cov ntu hauv qab no:
(1) TACH TSA:
Taag theem yog cov khoom ntim hauv lub computer tom nyiag sab, siv rau adsorb cov khoom sib xyaw. Cov theem nyob ruaj khov muaj feem cuam nrog silica gel, alumina, ua kom zoo dua cov roj carbon, thiab lwm yam kev sib txuas lus lossis kev sib cuam tshuam lub cev nrog cov khoom sib xyaw.
(2) piv ntaus ntawv loading ntaus:
Cov qauv loading ntaus ntawv yog siv los qhia cov qauv sib xyaw kom sib cais rau sab nraud ntawm sab nraud. Cov txheej txheem kev thauj cov txheej txheem coj los ntsuas cov koob txhaj tshuaj, Pipette chaw thau khoom, thiab lwm yam.
(3) Eluent sau ntaus ntawv:
Cov khoom ntim khoom sau tau yog siv los sau Eluent Eluent ntws tawm ntawm lub computer Tom qab kev tshawb xyuas kom paub ntxiv thiab nrhiav kom tau. Cov khoom siv sau ua ke suav nrog cov flatical flasks, kev kuaj hlab, thiab lwm yam.
(4) Nrhiav Kom Tau Ib Yam:
Cov kev tshawb pom yog siv los tshawb nrhiav thiab txheeb xyuas cov sib cais. Cov kev kuaj pom ib txwm muaj xws li UV pom spectroscopy, cov tshuaj tua kab mob fluorrometry, thiab cov tshuaj tua tsiaj tuaj yeem muab cov ntaub ntawv hais txog kev sib cais thiab kev huv ntawm cov khoom siv.
Hom
CC ua ib daim ntawv tseem ceeb ntawm chromatographic thev naus laus zis, yog siv dav hauv ntau daim teb thiab cov chemical, thiab tshuaj lom ib puag ncig, thiab kws tshuaj. Lub ntsiab cai tseem ceeb yog siv qhov sib txawv hauv kev faib cov coefficients ntawm cov sib xyaw ua ke, thiab ua tiav cov khoom sib cais los ntawm kev xaiv tsa ntawm cov mobile theem. Muaj ntau homkem chromatography khoom, uas tuaj yeem muab cais ua ntau hom raws li cov khoom siv, sib cais tshuab, thiab cov teb ntawm cov theem nyob ruaj khov. Cov hauv qab no yog cov ncauj lus kom ntxaws ntawm cov khoom lag luam.
Kev faib tawm raws li theem theem morphology
1. Sau kem
Ib kem ntawv ntim yog hom cc feem ntau yog sau los ntawm kev sau cov theem ruaj khov rau hauv cov lumen ntawm kab ntawv. Theem nyob ruaj khov tuaj yeem yog cov khoom siv khov kho, cov gel lossis polymer. Cov ntawv ntim tau muaj qhov siab siab thiab sib cais muaj peev xwm, tab sis tus thawj siab siab yog qhov siab, uas yuav tsum muaj kev huv ntawm cov qauv.
(1) Silicone kem: Silicone yog cov khoom siv uas siv tau dav hauv CC vim nws cov tshuaj ruaj khov zoo thiab muaj zog adsorption. Silica gel txhua yuav siv tau rau cais cov tebchaw polar, tshwj xeeb tshaj yog rau kev sib cais thiab huv cov organic sib txuas.
(2) Aluminium Oxide Pos: Aluminium Oxide kuj yog cov khoom siv uas siv tau. Piv nrog silica gel, nws muaj cov kev ua kom muaj zog tshaj tawm kev ua tau zoo thiab yog qhov haum rau kev sib cais sib txuas nrog cov muaj zog polarity. Alumina kem feem ntau siv rau kev rho tawm thiab sib cais ntawm cov khoom ntuj.
(3) Daim Ntawv Polymer: Cov khoom lag luam ntawm cov khoom siv polymer yog polymer, uas muaj cov tshuab muaj zog thiab tshuaj ruaj khov. Cov kab polymer polymer yog qhov tsim nyog rau kev cais cov pa hluav taws loj xws li cov protein, polysaccharides, thiab lwm yam.
2. Capillary Kem
Ib kem capillary yog ib qho qauv ua yeeb yaj kiab nyias nyias tsim los ntawm txheej ib theem nyob ruaj ruaj nyob rau sab hauv phab ntsa ntawm lub capillary raj. Capillary kab ntawv muaj feem pua qis dua thiab kev sib cais ntau dua, ua rau lawv haum rau kev tsom xam ntawm txoj kab kev kuaj xyuas. Cov capillary txhua feem ntau siv nyob rau hauv roj computers posraphized tomography thiab siab-ua kua hauv computer.
(1) Roj computerized tom qab caprograry capillary capillary capographized tom qab caprograry caprograry capograry capograry feem ntau yog cov qauv molecular, ion txauv thiab lwm cov hauv paus ntsiab lus. Roj roj computers tom qab caprographed capillary yog siv dav rau kev tsom xam hauv liaj teb xws li khoom noj, ib puag ncig, petrochemicals, thiab nkev.
(2) capillary capillary rau kua computerized tompographized Tomographized seem rau lub computer capographized tuaj yeem ua kua, uas haum rau kev sib cais thiab tshuaj ntsuam ntawm cov kab ua kua. Kua kua computer ua kua muaj ntau cov kev siv hauv kev tshuaj ntsuam xyuas, biomolecule sib cais, thiab lwm thaj teb.
Kev faib tawm raws li cais cov tshuab
1. Adsorption Cab chromatography
(1) Qhab Adsorption Koos: Cov theem siab ntawm polar adsorption kab ntawv yog tsim nyog rau kev sib cais polar, phenols, acids, thiab lwm yam.
(2) Tsis yog Polar Adsorption Kem: Theem Tsis Yog-Polar Adsorption yog qhov uas tsis yog cov pa roj carbon monoxide
2. Ion txauv kem chromatography
Ion Txauv CC Siv Oons Txauv Khoom Siv Ua Ntej Nyob Ruaj Ntseg Ua cais cov khoom sib xyaw los ntawm ion pauv pauv. Theem nyob ruaj khov ntawm ion txauv sab lub computer muaj ion pauv kev ua haujlwm, thiab lwm yam, cov khoom noj muaj txiaj ntsig, thiab lwm yam tshuaj hauv dej.
Tso rau ntawm daim ntawv thov teb
(1) Npaj sab chromatography
Npaj CC feem ntau yog siv rau kev sib cais thiab cov kev ua kom zoo ntawm cov qauv uas tom qab npaj lub computer tas li thiab npaj tiav lub koos pij tawj Tom. Cov kab uas hla ntawm kev npaj CC yog tus loj dua, uas tuaj yeem tiv thaiv cov khoom siv siab thiab txaus rau cov qauv kev sib cais thiab ua kom huv si hauv kev lag luam nplai.
(2) Kev ntsuas phoo chromatography
Analytical CC feem ntau yog siv rau cov qauv tshuaj xyuas thiab kev txheeb xyuas kom ua kua roj hmab ua kua (HPLC). Kev tshawb nrhiav CC muaj cov khoom me me uas muaj cov khoom siv sib xyaw ua ke, thiab kev sib cais ntau dua rau kev sib cais thiab tsom xam ntawm trace kuaj. AnalyTical CC feem ntau siv nyob rau hauv fields xws li kev soj ntsuam tshuaj yeeb thiab kev soj ntsuam ib puag ncig.
TSHWJ XEEB HOM PHIAJ LOS NQIS FOOGRITER TAYODY Cov Khoom Siv
Ncov Qaumteb hauv qab los ntawm kev sib cuam tshuam ntawm polar nyob ruaj ruaj theem thiab loj polar mob theem rau kev sib cais. Cov theem nyob ruaj khov ntawm sab qaum teb sab hauv qab zib yog ib qho tshuaj polar, xws li Silica Gel, Alumina, thiab lwm yam; Lub xov tooj mobile feem ntau yog ib qho kev siv roj av, cov pa roj polarographic tau haum rau kev sib xyaw ua ke hauv cov tshuaj tua kab mob hauv cov etortry, acid everispor, thiab lwm yam teb.
Tag
Muaj ntau cov cuab yeej siv ntau yam, uas tuaj yeem muab cais ua ntau hom raws li kev morphology ntawm cov theem ruaj ruaj, kev sib cais tshuab, thiab cov teb. Txhua yam khoom siv muaj nws cov yam ntxwv tshwj xeeb thiab kev thov cov khoom. Xaiv cov khoom tsim nyog yog qhov tseem ceeb heev rau kev ua tiav thiab sib cais raug thiab kev ua kom huv. Nrog kev nce qib nro ntawm science thiab thev naus laus zis thiab kev nthuav dav ntawm daim ntawv thov ua ke, kev txawj ntse, muab kev txhawb nqa ib puag ncig, muab kev txhawb nqa ib puag ncig rau kev tshawb fawb thiab kev tsim khoom.
Kev tiv thaiv tus kheej
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Thaum siv cov khoom siv no, kev tiv thaiv kev tiv thaiv tus kheej yuav tsum muaj los xyuas kom muaj kev nyab xeeb ntawm cov sim. Cov lus qhia tiv thaiv tus kheej hauv qab no yog ua raws cov kev cai sim kev nyab xeeb thiab cov kev paub tseeb:
Kev Nyab Xeeb Kev Nyab Xeeb Yuav tsum tau hnav thaum cov khoom siv chromatography los tiv thaiv cov khoom siv chromatography los tiv thaiv cov qauv chromaters xws li cov qauv, cov kuab tshuaj lossis silica gel hmoov. Kev tiv thaiv kev nyab xeeb yuav tsum tau raug xaiv raws li cov qauv kev nyab xeeb, thiab xyuas kom lawv cov khawb hauv.
Daim npog ntsej muag: Raws li cov xwm txheej tshwj xeeb ntawm kev sim, xaiv daim npog uas tsim nyog rau kev tiv thaiv kev ua pa. Yog tias sim sim cuam tshuam nrog kev tso tawm cov pa lom lossis phom sij, daim npog roj lossis nqus dej yuav tsum hnav. Rau silica gel hmoov av thiab lwm yam lus, koj tuaj yeem xaiv daim npog qhov ntswg rau kev tiv thaiv.
Cov hnab looj tes tiv thaiv: hnav cov hnab looj tes tiv thaiv kev tiv thaiv uas tsim nyog los tiv thaiv koj txhais tes los ntawm kev sib chwv nrog cov qauv kev raug mob, cov kuab tshuaj lossis silicone. Cov khoom siv hnab looj tes yuav tsum tau xaiv raws li cov kev xav tau ntawm kev sim, xws li looj hnab looj tes yas, nitrile cov hnab looj tes tiv thaiv.
Kev ua lub tsho tiv thaiv (lab) hnav lub tsho tiv no tshwj xeeb los tiv thaiv lub cev los ntawm cov tshuaj muaj teeb meem xws li cov qauv thiab cov kuab tshuaj. Chav ntxhua khaub ncaws yuav tsum huv thiab huv si, thiab hloov pauv tsis tu ncua thiab ntxuav.
Cov khau kev nyab xeeb: Hnav tsis-muag, tiv thaiv cov khau kev nyab xeeb kom tiv thaiv kev raug mob ko taw thaum sim. Tshwj xeeb tshaj yog nyob rau hauv kev tuav cov khoom ntawm cov khoom hnyav lossis cov kev sim ua rau cov khau ua rau kev nyab xeeb yog qhov tseem ceeb tshwj xeeb.
Daim npog lub ntsej muag: nyob rau qee kis, xws li thaum siv cov tshuaj tiv thaiv corrosive, nws yuav tsim nyog los tiv thaiv cov tshuaj tiv thaiv lub ntsej muag los tiv thaiv kev raug mob rau lub ntsej muag.
Cov kev tiv thaiv kev tiv thaiv: Yog tias muaj suab nrov hauv chav kuaj, koj yuav tsum hnav cov pob ntseg sib cav lossis pob ntseg pob ntseg.
Cov kev ceev faj
Kev khaws cov khoom tiv thaiv tus kheej: Cov cuab yeej siv tus kheej yuav tsum muab khaws cia kom zoo hauv qhov chaw tsim qauv thiab khaws kom huv thiab qhuav. Cov cuab yeej tiv thaiv tus kheej yuav tsum tau kuaj xyuas kom tiav thiab ua tiav ua ntej siv.
Kev hloov chaw ntawm cov cuab yeej tiv thaiv tus kheej: yog tias cov khoom tiv thaiv tus kheej tau raug puas tsuaj, tsis raug lossis tsis raug, koj yuav tsum hloov cov khoom tiv thaiv tam sim no.
Kev cob qhia kev kuaj cov neeg ua haujlwm: Cov neeg ntiav yuav tsum tau txais kev nyab xeeb kev nyab xeeb thiab paub meej tias lawv tuaj yeem hnav thiab siv cov khoom tiv thaiv tus kheej kom raug thaum lub sijhawm sim.
Txhawm rau suav, thaum siv cov khoom siv, cov cuab yeej yuav tsum hnav cov hnab looj tes tiv thaiv tus kheej, cov hnab looj tes, thiab xaiv lwm cov kev tiv thaiv kev tiv thaiv raws li cov kev sim tshwj xeeb. Nyob rau tib lub sijhawm, cov kws sim yuav tsum tau txais kev pab kev nyab xeeb kom ntseeg tau tias lawv tuaj yeem hnav thiab siv cov khoom tiv thaiv tus kheej kom ntseeg tau tias lawv tus kheej kev nyab xeeb.
Cim npe nrov: Kab cov khoom siv chromatography, Tuam Tshoj kem cov khoom siv chromatography, lwm tus neeg, lub Hoobkas
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