Protein ib chromatography Sab
2.Chromatogratographic Kem (Hloov Hom)
3.Chromatographic Tag (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
A Cov neeg muaj proteina cHROMATGODEctus hlisyog ib kem sib cais siv los ua kom huv cov protein uas siv cov protein uas muaj kev sib xyaw ua ke rau immunogism (IGG) kom cais iGg-nplua nuj sib tov los ntawm lwm cov impurities. Cov Protein A yog cov kab mob uas muaj cov kab mob uas muaj qhov siab thiab tshwj xeeb rau FC ib feem ntawm cov tshuaj tiv thaiv kab mob, tshwj xeeb tshaj yog IGG tiv thaiv.
Cov Protein feem ntau siv los ua kom huv IGG thiab lwm cov protein los ntawm ntshav qab zib, thiab yog ib qho ntawm cov txheej txheem molecular thiab biopharmaceutical.
Lub thermo scienceific pierce ™ cov protein ntau cov chromatographic purification kem, yog npaj-rau-siv cov khoom siv npaj ua ntej thiab cov tshuaj tiv thaiv monoclonal thiab monoclonal antibodies. Cov kab puv ntoob yog tsim nyog rau kev ua kom ua rau kev ua kom ua rau kev ua kom ua polyclonal thiab monoclonal IGG los ntawm ntshav dej, ASCites, thiab Cellites, thiab cellites kab lis kev cai. Cov khoom nta muaj protein ua ib, agarose resin kev txhawb nqa, inert, yooj yim, yooj yim, rov ua dua cov chromatographic sib cais, thiab yog txuas ntxiv rau ntau yam siv.
Chaw ua uas tsis tau haus
Cov yam ntxwv thiab cov txiaj ntsig
Raws li cov cuab yeej tseem ceeb hauv BIOS Deptopation Technology, tshwj xeeb tshaj yog nyob rau hauv kev tiv thaiv protein nyob rau hauv nws cov yam ntxwv tshwj xeeb thiab qhov tseem ceeb. Hauv qab no yog kev tsom xam cov yam ntxwv zoo thiab cov txiaj ntsig ntawm cov protein rau tag nrho cov txiaj ntsig hauv kev tshawb fawb Bioscientific thiab kev lag luam.
Txheej txheem cej luam ntawm cov yam ntxwv
High affinity thiab qhov tshwj xeeb
Cov tub ntxhais ntawm cov protein ib kem yog nws cov npoo ruaj khov ib qho molecule, uas muaj kev sib txawv thiab tshwj xeeb rau FC ntu ntawm immunogulin Igg. Cov cuab yeej no ua rau cov protein cov protein rau ib qho kom txuag tau thiab kho mob hlwb, cov tshuaj tsw qab thiab tsis zoo.
Kev Siv Feem
Cov protein ib kem yog tsis tsuas yog tsim nyog rau kev ua kom huv ntawm iGG los ntawm cov tsiaj, thiab tseem muaj cov tshuaj pleev ib txwm muaj los ntawm cov tshuaj pleev plab lossis cov tshuaj polyclon. Tsis tas li ntawd, nws tseem haum rau kev sib cais ntawm iGG lwg me me nrog hnyav molecules, subtypes thiab them nws cov peev xwm.
Kev sib cais ua tau zoo thiab ua kom huv
Ua tsaug rau lub siab Affinity ntawm cov neeg muaj protein ntau ntawm cov protein a thiab iGG, cov protein ib qho tuaj yeem ua tiav cov voj voog purification thiab txhim kho ntau dua kev ua tau zoo. Nyob rau tib lub sijhawm, los ntawm kev ua kom zoo dua cov xwm txheej kev tshem tawm, kev rov qab los ntawm IGG tuaj yeem txhim kho cov qauv kev tshawb fawb txog kev tshawb fawb thiab kev tsim khoom.
Zoo ruaj khov thiab ruaj khov
Protein ib hom feem ntau siv cov ntaub ntawv zoo li qub, xws li agarose, muaj ntau yam tshuaj lom neeg mob siab, kom ntseeg tau tias ntev ruaj khov thiab siv tau ntev.
Cov ncauj lus kom ntxaws piav qhia ntawm cov txiaj ntsig
Txhim kho kev ua kom huv si
Lub siab sib xws thiab tshwj xeeb ntawm cov protein ib qho kev ua kom tiav cov txheej txheem ntawm IGG ntau dua, thiab lub sijhawm txo qis cov kev ua kom tiav. Nyob rau tib lub sij hawm, lub siab siab dawb thiab siab rov qab ntawm IGG cov khoom kuj muab kev ntseeg siab lees paub rau kev sim ua neeg muaj kev txhim kho tshiab thiab kev tsim kho.
Txo cov nqi ntau lawm
Vim yog lub durability thiab rov ua dua cov protein ib kem, tus nqi ntawm ib leeg purification yog tswj tau zoo. Ib qho ntxiv, los ntawm kev tsim kho cov txheej txheem kev ua haujlwm thiab cov txheej txheem kev tsim kho tshiab, cov kev pabcuam lub neej tuaj yeem txuas ntxiv thiab tus nqi tsim khoom tuaj yeem txo qis dua.
Xyuas kom cov khoom lag luam zoo
Qhov siab tshwj xeeb thiab kev ua kom siab purity lav ntawm cov protein ib qho kev lim hiam thiab kev ruaj khov rau cov kev siv tshuaj ntsuab thiab kev siv tshuaj tiv thaiv tshuaj thiab kev siv tshuaj tua kab mob.
Yooj yim rau kev khiav lag luam thiab tswj hwm
Niaj hnub protein ib qho qauv tsim feem ntau yog siv cov neeg siv kev ua phooj ywg, thiab lwm yam kev ua haujlwm yooj yim dua thiab sai dua. Nyob rau tib lub sijhawm, cov kev txhawb nqa cov kev txhawb nqa rov qab thiab cov txheej txheem kuj tseem muab kev yooj yim rau kev saib xyuas thiab saib xyuas ntawm kem.
Tag
Hauv cov ntsiab lus, cov Protein ib qho kev tshawb fawb Chromatographic ua rau muaj kev tshawb fawb txog kev ua kom zoo, ua kom muaj txiaj ntsig zoo, ua haujlwm rau kev coj ua tau zoo thiab yooj yim. Nrog rau kev nce qib txuas ntxiv ntawm biotechnology thiab kev ua tau zoo tshaj plaws, muab cov kev daws teeb meem ntxiv rau ntau qhov kev tshawb fawb thiab kev thov.
Daim ntawv thov ntawm Purified immunoglobulin (IGG)
Daim ntawv thov ntawm cov protein ib hauv phurification ntawm IGG
Ua Purification Zoo Tshaj:
Cov protein ib qho tshwj xeeb heev thiab xaiv, ua kom muaj kev sib cais ua kom zoo thiab huv ntawm IGG los ntawm cov mixatures.
Txawm hais tias IGG yog muab rho tawm los ntawm ntshav dej hiav txwv, ntshav lossis kab lis kev cai ntawm tes, protein ib kem muab cov khoom siv purity.
Haum rau ntau hom tsiaj
Protein ib kem yog tsis tsuas yog tsim nyog rau tib neeg lub cev tsis haum, tab sis kuj tsim nyog rau luav, tus npua, miv, miv, miv thiab lwm yam tsiaj muaj tsiaj.
Qhov no ua rau cov protein ib qho ntau ntawm cov ntawv thov muaj nuj nqis hauv kev sim tsiaj, kev txhim kho tshuaj thiab kev kuaj mob.
Yooj yim rau kev khiav lag luam
Niaj hnub muaj protein niaj hnub siv tau npaj-rau-siv prepacking khoom siv, uas yog qhov yooj yim thiab ceev ceev rau kev khiav lag luam.
Cov neeg siv tuaj yeem tau txais cov kev siab purity higg los ntawm kev ua raws li cov txheej txheem ua ntej yooj yim thiab cov kauj ruam uas tsis lees paub hauv cov lus qhia.
Rov Ua Dua thiab Ruaj Khwv
Cov protein ib kem muaj cov kev ua kom siab dua thiab ruaj khov, uas tuaj yeem ua kom cov txiaj ntsig zoo ib yam ntawm txhua qhov kev sim ua kom huv.
Qhov no yog qhov tshwj xeeb tshaj yog qhov tseem ceeb rau kev thov cov xwm txheej uas xav tau loj ntau lawm thiab kev tswjhwm zoo.
Protein ib kab ntawv xaiv thiab kev ceev faj
Hom Xaiv
Thaum xaiv cov protein ib qho, nws yog qhov tsim nyog los xav txog kem muaj peev xwm, ntws tus nqi, siab poob thiab lwm yam kev ntsuas kom ntseeg tau tias qhov kev xav tau ntawm kev sim lossis kev tsim khoom tau ua tiav.
Nyob rau tib lub sijhawm, nws kuj tseem yuav tsum xav txog qhov muaj peev xwm thiab kev pabcuam lub neej ntawm kem los txo cov nqi thiab txhim kho kev ua tau zoo.
Kev Ceev Kev Ua Haujlwm
Ua ntej siv cov protein ib kem, cov ntawv yuav tsum tau ua kom pom tseeb thiab sib npaug.
Thaum lub sijhawm thauj khoom, cov dej ntws uas tsim nyog thiab siab yuav tsum tau tswj kom tsis txhob thaiv lossis puas rau kem.
Thaum lub sijhawm ua tiav cov txheej txheem, cov neeg muaj peev xwm kom tsim nyog thiab raug xaiv tau xaiv kom ntseeg tau tias lub IGG tuaj yeem raug kaw kiag li los ntawm sab.
Tom qab siv, cov ntawv yuav tsum tau huv thiab khaws cia kom ntev nws lub neej pabcuam.
Qhov zoo thiab cov nyom ntawm cov protein hauv kem hauv IGG Purification
Qhov Zoo:
High efficiency, feem ntau thiab kev xaiv.
Haum rau kev ua kom huv ntawm ntau hom tsiaj muaj tsiaj
Yooj yim thiab nrawm kev ua haujlwm, ua kom rov ua dua thiab ruaj khov.
Sib tw:
Tus nqi ntawm cov protein yog tus kheej yog qhov tseem ceeb, tshwj xeeb tshaj yog rau cov pawg ntau lawm.
Hauv qee kis, xws li thaum muaj kev cuam tshuam ntau ntawm kev ua kom muaj zog lossis cov kauj ruam, cov kauj ruam ntxiv tau los txhim kho kev ua kom muaj kev ua kom muaj kev ua kom muaj txiaj ntsig.
Daim ntawv thov hauv kev txhim kho thiab kev tsim cov tshuaj tiv thaiv tshuaj tiv thaiv
Daim ntawv thov ntawm cov protein hauv ib sab hauv kev tsim tshuaj tiv thaiv kab mob tshuaj tiv thaiv
Tshuaj Txhuam Tshuaj Ntsuam Xyuas thiab Qhia Txog
Nyob rau hauv cov theem kev txhim kho thaum ntxov ntawm cov tshuaj tiv thaiv tshuaj tiv thaiv kab mob, cov protein tuaj yeem siv los tshuaj ntsuam thiab txheeb cov tshuaj tiv thaiv kab mob tshwj xeeb thiab tshwj xeeb. Los ntsuas kev ntsuas lub peev xwm ntawm cov tshuaj tiv thaiv ntawm cov tshuaj tiv thaiv rau cov protein rau ib qho, qhov muaj peev xwm ua tau zoo thiab ua kom muaj zog thiab kev tshuaj tiv thaiv tau ua tiav.
Tsis tas li ntawd, cov Protein ib qho yuav siv los txiav txim siab cov cell clones zoo tshaj plaws, piv txwv li cov uas tuaj yeem hais cov tshuaj tiv thaiv kab mob zoo tshaj plaws.
Tshuaj Tiv Thaiv Cov Tshuaj Tiv Thaiv
Thaum lub sij hawm cell kev coj noj coj ua, cov protein ib los soj ntsuam cov tshuaj tivthaiv cov tshuaj tivthaiv cov tshuaj tiv thaiv kab mob hauv cov cell kab lis kev cai supernatant. Qhov no yog qhov tseem ceeb kom txiav txim siab lub sijhawm sau npe zoo rau kom ntseeg tau tias muaj ntau qhov kev tiv thaiv thiab cov tshuaj tiv thaiv zoo tau sau.
Piv txwv li, cov tskgel protein -5 Pw cov lus tsis raug thiab muaj qhov siab nrawm, muaj qhov siab tshaj plaws thiab qhov tseeb.
Ua kom zoo ntawm cov txheej txheem tiv thaiv antibody purifications
Cov Protein tau siv los ua kom ua kom zoo rau cov txheej txheem tiv thaiv antibody cov txheej txheem xws li ntas tsis sib xws, Ph, lub zog muaj zog thiab txaus. Los ntawm kho cov mob no, cov kev coj dawb huv thiab tawm los ntawm cov tshuaj tiv thaiv tuaj yeem txhim kho, thiab tus nqi tsim khoom tuaj yeem txo.
Ib qho ntxiv, cov Protein ib qho yuav siv los ntsuas qhov ua tau zoo ntawm cov kev ua kom muaj kev ua kom muaj cov txheej txheem kev txhim kho cov txheej txheem thiab kev txhim kho cov txheej txheem.
Daim ntawv thov ntawm cov protein cov chromatography nyob rau hauv kev tsim cov tshuaj tiv thaiv tshuaj tiv thaiv
Tshuaj tiv thaiv kab mob:
Hauv kev tsim cov tshuaj tiv thaiv kab mob tshuaj tiv thaiv kab mob, cov protein feem ntau siv los ua cov cuab yeej ntawm kev xaiv rau cov kauj ruam ntes. Nrog cov kauj ruam no, cov tshuaj tiv thaiv phiaj tuaj yeem ntes tau los ntawm cell kev coj noj coj ua thaum tshem tawm feem ntau impurities.
Piv txwv li, cov agilent cov agilent bio-monolith protein ib sab thiab bleinker's chromax ant are ant20 antigody ntes cov kauj ruam thiab yog tus cwj pwm los ntawm kev xaiv siab, kev xaiv thiab kev xaiv.
Tshuaj Tiv Thaiv Kev Ua Yeeb Yam:
Thaum lub sij hawm cov protein ib qho chaw ua yeeb yam zoo nyob rau hauv cov kauj ruam tiv thaiv kab mob, qee cov kauj ruam ntxiv rau cov kauj ruam kev coj dawb huv thiab kev sib txuam ntawm cov tshuaj tiv thaiv kab mob. Txawm li cas los xij, nws yog ib qho tseem ceeb kom nco ntsoov tias qee cov kev ua kom muaj kev tsim nyog siv tau siv lwm hom ntawm cov chromatography lossis hydrophobic sib cuam tshuam cov chratography.
Txawm li cas los xij, cov Protein ib qho yuav siv tau ua ib qho cuab yeej siv hluav taws xob xws li cov protein ntau xws li cov protein ntau dua li cov protein, HCP), DNA, thiab kab mob.
Cov txheej txheem sib tw thiab siv tau:
Protein ib qho tseem ua lub luag haujlwm tseem ceeb hauv theem teev thiab siv tau theem ntawm cov txheej txheem tshuaj tiv thaiv kev siv tshuaj tiv thaiv tshuaj. Cov kev ua tau zoo thiab kev ruaj khov ntawm cov protein ib qho kev tsim tawm Chromatographic hauv ntau ntau lawm tuaj yeem ntsuas los ntawm cov txheej txheem nplai simulating ntau lawm.
Tsis tas li ntawd, kev sib raug zoo thiab kev ntseeg tau ntawm cov protein ntau hauv cov txheej txheem tsim khoom, uas muab cov tshuaj tiv thaiv kev lag luam tshuaj tiv thaiv tshuaj tiv thaiv.
Daim Ntawv Thov thiab Prospect
Daim ntawv thov: Protein lub chromatography Sab yog dav siv hauv kev txhim kho tshuaj thiab kev tsim tshuaj tiv thaiv tshuaj, thiab tseem siv hauv kev tshawb tshuaj lom neeg roj ntsha thiab kuaj mob.
Cov zeem muag: nrog kev nthuav dav txuas ntxiv ntawm cov tshuaj tiv thaiv tshuaj thiab kev thov cov protechnology, qhov kev thov rau cov protein me me yuav txuas ntxiv ntxiv. Nyob rau tib lub sijhawm, rov tshwm sim ntawm cov sau ntawv tshiab thiab cov tswv yim Chratographic tseem yuav tseem muaj cov protein montomatogrogress.
Cim npe nrov: Protein ib cov chromatography Sab, Tuam Tshoj protein ib cov chromatography Sign Manufacturers, lwm tus neeg, Hoobkas
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