Methanol puas tuaj yeem ua Rotovapped?
Apr 13, 2024
Tso lus
Yog lawm,methanol cYuav tsum tau khiav tawm siv lub rotating evaporator, feem ntau alluded ua ibrotovap ua. Lub evaporator revolving yog ib qho cuab yeej tshawb fawb siv los tshem tawm cov kuab tshuaj los ntawm kev npaj los ntawm dissipation hauv qab qhov hnyav poob thiab tswj qhov kub. Methanol, ua ib qho tsis ruaj tsis khov uas tsis muaj kev cuam tshuam nrog feem ntau moo bubbling point (64.7 degree lossis 148.5 degree F), tuaj yeem raug tshem tawm thiab khiav tawm ntawm kev npaj siv rotovap.
Kev Npaj Kev Npaj
Cov txheej txheem uas muaj methanol yog muab tso rau hauv ib puag ncig-bottomed carafe, uas yog nyob rau ntawm qhov chaw koom nrog lub rotating evaporator.
Kev siv lub tshuab nqus tsev vacuum
Lub moj khaum yog tsau, thiab lub tshuab nqus tsev twj yog siv los txo qhov hnyav sab hauv lub thawv. Qhov no ua rau lub bubbling point ntawm methanol, tso cai rau nws mus ploj ntawm qhov kub thiab txias.
Cua sov
Kev npaj nyob rau hauv lub hub yog maj mam warmed, txawm nrog ib tug da dej los yog ib tug sov so mantle, kom nce tus nqi ntawm vanishing. Qhov kub thiab txias yog ua tib zoo tswj kom cia siab tias overheating lossis debasement ntawm qhov ntsuas.
Condensation
Raws li methanol evaporates los ntawm cov tshuaj, nws nce mus rau hauv lub condenser, qhov twg nws yog txias thiab condensed rov qab mus rau hauv cov kua daim ntawv. Cov condensed methanol yog sau nyob rau hauv ib tug nyias muaj nyias lub raj mis.
Kev sau cov residue:Cov tshuaj ntxiv nyob rau hauv lub khob ntim hauv qab, tam sim no depleted ntawm methanol, yog concentrated raws li cov kuab tshuaj raug tshem tawm. Cov khoom uas xav tau los yog cov khoom yuav raug tso tseg hauv lub khob.
Ntxuav thiab khaws cia:Tom qab cov txheej txheem tiav lawm, cov cuab yeej raug tshem tawm, thiab cov khoom siv methanol tuaj yeem muab pov tseg kom zoo lossis rov siv dua yog tias xav tau. Cov cuab yeej raug ntxuav thiab khaws cia rau yav tom ntej siv.
Nkag siab txog Rotary Evaporation
Rotary evaporation, feem ntau hu ua rotovap los yog rotavap, yog cov txheej txheem siv dav hauv cov chaw soj ntsuam thiab kev lag luam rau kev tshem tawm cov kuab tshuaj los ntawm cov qauv ua kua. Nws yog ua raws li lub hauv paus ntsiab lus ntawm evaporation nyob rau hauv txo lub siab thiab tswj qhov kub thiab txias kom ua tau zoo thiab xaiv cov kuab tshuaj cais tawm ntawm cov khoom sib xyaw uas xav tau. Ntawm no yog ib qho kev sib faib ntawm cov rotary evaporation ua haujlwm li cas:
Kev teeb tsa:Lub rotary evaporator muaj ntau yam tseem ceeb:
Rotating Flask:Qhov no yog lub nkoj uas cov qauv ua kua, uas muaj cov kuab tshuaj tshem tawm, muab tso rau. Nws feem ntau yog ib lub khob ntim hauv qab uas tuaj yeem tig los txhim kho evaporation.
Dej los yog roj da dej:Lub raj mis zaum hauv dej kub los yog roj da dej, muab cov cua sov zoo rau cov qauv.
Rotating Evaporating Flask:Tag nrho lub raj mis sib dhos, suav nrog cov qauv, yog tig los ua kom cov cheeb tsam nthuav tawm thiab ua kom yooj yim evaporation.
Condenser:Lub condenser yog txuas rau lub hwj kom txias thiab condense cov evaporated hnyav rov qab mus rau hauv cov kua daim ntawv. Nws tiv thaiv cov kuab tshuaj vapors los ntawm kev khiav mus rau hauv qhov chaw.
Lub tshuab nqus tsev twj:Lub tshuab nqus tsev twj tso kua mis yog siv los txo qhov siab hauv qhov system, txo qhov kub ntawm qhov hnyav thiab ua kom nrawm evaporation.
Kev siv lub tshuab nqus tsev vacuum:Lub kaw lus kaw, thiab lub tshuab nqus tsev twj tso kua mis yog qhib los tsim lub tshuab nqus tsev hauv lub hwj. Qhov no txo qhov siab, txo qhov kub ntawm qhov hnyav. Piv txwv li, ntawm qhov txo qis, qhov kub ntawm cov dej txo los ntawm 100 degree (212 degree F) ntawm tus qauv atmospheric siab kom qis dua.
Cua sov:Cov dej los yog roj da dej yog rhuab mus rau qhov kub thiab txias me ntsis hauv qab lub boiling point ntawm qhov hnyav. Cov cua sov maj mam ua kom cov qauv evaporates maj mam thiab sib npaug yam tsis muaj cua sov lossis degradation ntawm cov khoom xav tau.
Evaporation:Raws li cov qauv yog rhuab thiab lub siab txo, cov kuab tshuaj pib evaporate los ntawm cov kua sib tov. Lub rotating flask txhim kho qhov chaw ntawm lub tshuab nqus tsev, txhawb nqa evaporation zoo.
Condensation:Cov evaporated hnyav vapor nce mus rau hauv lub condenser, qhov twg nws yog txias thiab condensed rov qab mus rau hauv cov kua daim ntawv. Cov kuab tshuaj condensed sau rau hauv ib lub khob cais, lub npe hu ua lub khob tau txais.
Kev sau cov residue:Cov qauv seem nyob rau hauv lub khob rotating, tam sim no depleted ntawm cov kuab tshuaj, ua ntau concentrated thaum evaporation zuj zus. Cov khoom sib xyaw uas xav tau lossis cov khoom lag luam yuav raug tso tseg hauv lub khob rau kev ua haujlwm ntxiv lossis kev tshuaj xyuas.
Saib xyuas thiab tswj:Thoob plaws hauv cov txheej txheem, qhov tsis xws li qhov kub thiab txias, lub tshuab nqus tsev, thiab kev sib hloov ceev tau saib xyuas thiab kho raws li qhov xav tau los ua kom zoo dua qub thiab ua kom muaj kev nyab xeeb ntawm kev ua haujlwm.
Kev tu thiab tu:Thaum evaporation tiav lawm, lub tshuab yog disassembled, thiab cov khoom hnyav tuaj yeem muab pov tseg los yog rov siv dua. Cov khoom ntawm cov evaporator rotary yog ntxuav thiab khaws cia rau kev siv yav tom ntej.
Kev tsim nyog ntawm Methanol rau Rotary Evaporatio
Methanol, ib tug polar solvent nrog ib tug tsawg boiling point ntawm 64.7 degree, nthuav cov ntaub ntawv intriguing rau rotary evaporation. Nws cov khoom muaj txiaj ntsig zoo, xws li kev hloov pauv siab thiab miscibility nrog dej thiab ntau cov kuab tshuaj organic, ua rau nws yog tus neeg sib tw txaus nyiam rau cov txheej txheem tshem tawm hnyav. Txawm li cas los xij, qee yam tseem ceeb rau kev txiav txim siab ua ntej yuav raug methanol rau rotovapping.
Kev xav txog kev nyab xeeb
Ib qho kev txhawj xeeb tseem ceeb cuam tshuam nrog methanol yog nws cov tshuaj lom. Kev cuam tshuam rau methanol vapors lossis kev noj cov khoom me me tuaj yeem ua rau muaj kev noj qab haus huv hnyav, suav nrog kev dig muag thiab kev puas hlwb. Yog li, kev ntsuas kev nyab xeeb nruj yuav tsum tau ua thaum tuav cov tshuaj methanol hauv chav kuaj. Kev ua pa kom txaus, cov cuab yeej tiv thaiv tus kheej (PPE), thiab kev ua raws li cov txheej txheem kev nyab xeeb tsim nyog yog qhov tseem ceeb los txo cov kev pheej hmoo cuam tshuam nrog methanol raug.
Cov tswv yim xav hauv Rotovapping Methanol
Txawm hais tias nws toxicity, methanol tuaj yeem raug rau rotary evaporation raws li qhov tsim nyog. Txawm li cas los xij, qee qhov kev txiav txim siab yuav tsum tau coj mus rau hauv tus account kom ntseeg tau tias kev ua tau zoo thiab kev nyab xeeb ntawm cov txheej txheem. Ua ntej, nws raug nquahu kom ua rotovapping ntawm methanol nyob rau hauv lub fume hood los yog ib qho chaw zoo-ventilated kom txo tau cov vapors. Tsis tas li ntawd, kev siv lub tshuab nqus tsev evaporator nruab nrog lub tshuab nqus tsev twj tso kua mis muaj peev xwm tsim cov qib nqus tsev vacuum yog qhov tseem ceeb rau kev tshem tawm cov kuab tshuaj zoo. Tsis tas li ntawd, kev saib xyuas cov txheej txheem evaporation kom ze thiab tswj tsis tau xws li qhov kub thiab txias thiab lub tshuab nqus tsev yog qhov tseem ceeb los tiv thaiv kev sib tsoo los yog ua npuas ntau dhau, uas tuaj yeem cuam tshuam kev ncaj ncees ntawm kev sim.
Daim ntawv thov ntawm Methanol Rotovapping hauv Laboratory
Txawm hais tias nws cov kev sib tw, rotovapping ntawm methanol pom muaj ntau yam kev siv hauv chav kuaj. Los ntawm cov concentration ntawm botanical extracts thiab tej yam ntuj tso khoom mus rau lub purification ntawm synthesized tebchaw, methanol teb evaporation muaj ib tug ntau yam thiab npaum txhais tau tias ntawm hnyav tshem tawm. Tsis tas li ntawd, kev sib raug zoo ntawm methanol nrog ntau yam kev tshuaj ntsuam xyuas, xws li chromatography thiab spectroscopy, ntxiv dag zog rau nws cov txiaj ntsig hauv kev tshawb fawb hauv chav kuaj.
Xaus
Hauv kev xaus, thaum methanol ua rau muaj kev txhawj xeeb txog kev nyab xeeb vim nws cov toxicity, nws tuaj yeem ua rau cov evaporation nyob rau hauv kev tswj xyuas. Los ntawm kev ua raws li cov txheej txheem kev nyab xeeb nruj thiab siv cov cuab yeej tsim nyog thiab cov txheej txheem, cov kws tshawb fawb tuaj yeem siv cov txiaj ntsig ntawm methanol rotary evaporation nyob rau hauv ntau yam kev sim. Txawm li cas los xij, kev ceev faj yuav tsum tau siv los txo cov kev pheej hmoo cuam tshuam thiab ua kom muaj kev nyab xeeb ntawm cov neeg ua haujlwm. Nrog rau kev ua tib zoo saib xyuas thiab kev ua kom zoo, methanol rotovapping tseem yog ib qho cuab yeej tseem ceeb hauv arsenal ntawm cov kws tshuaj ntsuam xyuas.
Cov ntaub ntawv:
"Methanol Safety Data Sheet." Sigma-Aldrich. [https://www.sigmaaldrich.com/catalog/product/sial/34860?lang=en®ion=US]
Jochum, Thomas et al. "Kev nyab xeeb siv methanol hauv kev kawm." Analytical thiab Bioanalytical Chemistry, vol. 409, Nr. 25 Kaum Ib Hlis 2017, p. 5919-5920. [https://doi.org/10.1007/s00216-017-0489-2]
Kruve, Anneli et al. "Kev Tshawb Fawb Txog Kev Tshawb Fawb Txog Kev Ua Li Cas Chromatography-Mass Spectrometry Methods: Part I." Analytica Chimica Acta, vol. 870, 2015, p. 29-44 ib. [https://doi.org/10.1016/j.aca.2015.02.019]