Hydrophobic kem chromatography

Hydrophobic sib cuam tshuam chomatography(HIC) yog cov txheej txheem muaj zog siv nyob rau hauv kev sib cais thiab huv cov protein, tshwj xeeb yog cov uas muaj hydrophobic cov khoom. Qhov kev cuam tshuam rau cov chromatographic no cov kev sib txawv ntawm cov qauv hydrophobic ntawm cov qauv me me, ua kom muaj kev sib cais ntawm cov khoom siv raws li lawv txoj kev tsiv teb tsaws thaum muaj kev sib cais. Hauv cov ntawv sau thoob plaws, peb yuav hais rau hauv cov ntsiab cai, kev ua haujlwm, qhov zoo ntawm hydrophobic sib cuam tshuam chratography.

Lub hauv paus ntawm cov lus dag hauv hydrophobic sib txuas ntawm cov protein molecules thiab hydrothobic ligands txuas nrog theem chaw nyob ruaj ruaj. Proteins, ua Amphipathic nyob rau hauv qhov, muaj ob qho tib si hydrophilic thiab hydrophobic seem. Nyob rau hauv aqueous cov kev daws teeb meem, hydrophilic nyob sab nraud yuav ntsib sab nraud, thaum hydrophobic cov seem feem ntau faus nyob rau hauv cov protein tus qauv pab las. Txawm li cas los xij, raws li qee yam kev mob, xws li muaj ntsev ntau, kev cuam tshuam nrog hydrophobic ligands ntawm cov chromatographic matrix.

Cov mobile theem hauv HIC feem ntau muaj cov kua ntsev ua kom haum nrog lub pher ntau ntawm 6-8. Cov ntsev ntau cov ntsev ua haujlwm tau txhim kho cov kev sib txuas lus hydrophobic, pab txhawb cov kev khi ntawm cov protein rau theem nyob ruaj khov. Tawv txo cov ntsev ntawm cov ntsev nyob rau hauv lub xov tooj ntawm tes thaum muaj zog ua rau muaj zog ntxuav, tso cai rau cov protein kom raug rau lawv hydrothobicity. Proteins nrog kev sib txuas lus hydrophobic tsis muaj zog raug kaw ua ntej, ua raws li cov neeg muaj zog sib cuam tshuam.

Qhov txheej txheem ntawm hydrophobic kem chromatography cuam tshuam, suav nrog cov qauv kev ua piv txwv, chaw sib npaug, kev xaiv tsa, thiab sau cov feem.

◆ Kev Npaj Ua Li Cas:
Ua ntej thauj cov qauv rau hauv kem, nws yog qhov tseem ceeb los npaj ntsev kom phim rau cov ntsev theem A (Equilibrium tsis). Cov PHF ntawm cov tshuaj piv txwv yuav tsum tau hloov kho kom tau raws li cov qauv kev tshaj tawm.

Sab Soc Pequilibration:
Cov kab ntawv yog sib npaug nrog mobile theem A, uas yog qhov ua tau ntsev ua tiav ntawm cov kua thiab ntsev. Qhov no ua kom ntseeg tau tias cov theem ruaj ruaj yog cov av noo nrog cov tsis huv, npaj txhij los cuam tshuam nrog cov qauv protein.

Muab cov qauv:
Cov qauv npaj tau thauj mus rau hauv kem. Qhov ntim ntawm cov qauv yog cuam tshuam los ntawm cov kev sib xyaw ua ke thiab cov khoom siv muaj peev xwm ntawm cov xov xwm. Rau cov qauv ua haujlwm, chaw thau khoom ncaj qha yog qhov ua tau yam tsis muaj ua ntej muaj kev pom zoo.

◆ Kev lees paub:
Kev lees paub yog ua tiav los ntawm maj txo cov ntsev ntawm cov mobile theem, yog li tsis muaj zog ntawm cov protein thiab theem nyob ruaj khov. Lwm txoj kev, kev xaiv tsa tuaj yeem ua tiav los ntawm kev ntxiv cov kuab tshuaj organic los yog xab npum mus rau theem mobile los hloov nws cov protein.

Fulling ntawm cov leb:
Cov feem raug tshem tawm tau sau thiab tshuaj ntsuam los ntsuas cov kev coj dawb huv thiab rov qab ntawm cov hom phiaj cov protein.

Ob peb yam cuam tshuam ntau qhov kev sib cais thiab kev ua kom huv ntawm cov protein hauv Hydrophobic kem chromatography:

◆ Ntsig ntsev thiab hom:
Hom thiab concentration ntsev nyob rau hauv lub xov tooj ntawm tes ua si ib tug pivotal lub luag haujlwm hauv modulating hydrophobic sib txuas lus. Salts xws li ammonium sulfate thiab sodium tshuaj muaj feem ntau siv, nrog rau}. 75 txog 4 mol / l rau sodium chloride.

◆ Ph:
Cov PH ntawm cov mobile theem cuam tshuam rau lub xeev thiab hydrophobicity ntawm cov protein. Ib hom protein ntawm cov protein lub oolectric point nyhav kom nyiam kev lees paub los ntawm kev txo cov hydrophobic sib txuas lus.

◆ kub thiab txias:
Nce cov kab ntawv kub tuaj yeem txhim kho hydrophobic sib txuas lus, ua rau kom txhim kho kev sib cais.

◆ Ntog ntws mus:
Tus nqi txaus cuam tshuam lub sijhawm nyob hauv kem, cuam tshuam lawv kev sib cuam tshuam nrog theem nyob ruaj ruaj.

Cov Cwj Pwm Hauv Paus:
Qhov ntev, txoj kab uas hla, thiab cov khoom ntim ntawm cov kem tag nrho pab rau kev sib cais ua haujlwm. Cov kev xaiv ntawm cov khoom ruaj khov cov khoom siv thiab nws cov khoom saum npoo kuj tseem ceeb heev.

Tam sim no nrhiav cov ntawv thov muaj cov protein ntau, suav nrog cov nqaij tawv muaj protein, membrane-cov protein, cov receptors, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj ntsha, thiab cov roj r recomic. Nws maj mam cais tej yam kev mob ua rau nws tsim nyog rau cov tshuaj yeeb yaj kiab, xws li cov enzymnes, thiab lwm yam tshuaj tiv thaiv kab mob.

Qhov Zoo:

1) Tus nqi rov qab muaj siab: HIC muab cov protein ntau dua, ua rau nws muaj txiaj ntsig rau cov txheej txheem ua kom huv.

2) Tswj cov kev ua si protein: kev sib cais me me txo cov protein thiab ploj ntawm cov haujlwm.

3) Versility: HIC tuaj yeem hloov kho rau cov kev ua kom huv ntawm ntau cov protein nrog sib txawv hydrophobic zog.

Tsis zoo:

1) solubility tsawg: Qee cov protein yuav nthuav tawm solubility ntawm cov ntsev siab, lim cov kev siv hauv no.

2) Kev cuam tshuam ntsev: ntau cov ntsev ua ntu zus hauv cov theem mobile tuaj yeem cuam tshuam nrog cov txheej txheem tshawb nrhiav tom ntej, tsim nyog ntxiv cov txheej txheem desalting.

Tsis ntev los no kev nce qib hauv HIC tau tsom mus rau kev txhim kho ntawm cov tshiab chratomaphic matricescees nrog kev sib cais ua kom muaj kev sib cais zoo nkauj thiab kev ruaj ntseg. Kev koom tes ntawm hydrophilic sib cuam tshuam chrataphyraphy (hilic) cov ntsiab cai thiab kev siv cov resins hom tau nthuav tawm ntawm kev ua haujlwm ntawm kev sib cais ntawm cov ncej sib dhos.

Ntxiv mus, kev sib koom ua ke ntawm HIC nrog rau lwm cov tshuaj chromatographic, xws li ion-txig chromatography, tau ua kom yooj yim siv zog ua kom muaj zog thiab muaj zog ua kom muaj zog. Kev nce qib hauv Automation thiab High-topput screen technologies thev naus laus zis kuj tseem pab txhawb rau cov ntsuas kev ntsuas thiab rov ua haujlwm ntawm kev ua haujlwm hic.

Cov lus qhia yav tom ntej hauv kev tshawb fawb nrog kev tshawb nrhiav ntawm Lwm Tus Neeg Zoo thiab matrices los txhim kho kev sib cais efficiencies thiab nthuav dav cov hnoos qeev ntawm cov ntawv thov. Kev loj hlob ntawm ntau qhov ntau ntawm eco-friendly thiab kev ua kom zoo ntawm kev ua kom zoo ntawm cov mob uas tsis meej, nyob twj ywm ntawm cov kev tshawb fawb tsis tu ncua.

Hauv kev xaus, hydrophobic sib cuam tshuam cov chratasider sawv cev rau kev sib cais thiab ua kom huv ntawm cov protein, tshwj xeeb yog cov uas muaj hydrophobic cov khoom. Los ntawm kev siv zog ntawm hydrophobic sib cuam tshuam ntawm cov piv txwv cov qauv thiab cov protein ntau rau ntau yam kev kho mob, kuaj mob, thiab cov ntawv thov tshawb fawb. Nrog rau kev nce qib tsis tu ncua hauv cov ntaub ntawv chromatographic, kev ua lag luam, thiab kev sib xyaw ua ke txawm tias muaj cov txuj ci ntau dua thiab kev siv hluav taws xob hauv thaj teb ntawm Biopharmaceuticals.

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