Fungsi conical flask
1) nqaim-lub qhov ncauj fwj: 50ml ~ 10000ml;
2) be fwj loj: 50ml ~ 3000ml;
3) Horn qhov ncauj: 50ml ~ 5000ml;
4) Lub qhov ncauj dav dav: 50ml / 100ml / 250ml / 500ml / 1000mL;
5) Conical flask nrog npog: 50ml ~ 1000mL;
6) Ntsia Hlau Congical Flask:
a. Lub hau dub (cov teeb tsa dav dav): 50ml ~ 1000mL
b. Txiv kab ntxwv hau (tuab hom): 250ml ~ 5000ml;
2. Ib leeg thiab ntau lub qhov ncauj puag ncig lub hauv qab flask:
1) Tib qho lub qhov ncauj puag ncig cov flask: 50ml ~ 10000ml;
2) Inclined peb-lub qhov ncauj flask: 100ml ~ 10000ml;
3) Inclined plaub-qhov ncauj flask: 250ml ~ 20000ml;
4) ncaj peb-lub qhov ncauj flask: 100ml ~ 10000ml;
5) Ncaj plaub-qhov ncauj flask: 250ml ~ 10000ml.
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
Tusfungsi conical flask, feem ntau raug xa mus rau yooj yim raws li ib tug conical flask, yog ib qho ntau thiab lub chaw kuaj hluav taws xob tseem ceeb siv hauv cov microbiology, biotechnology, thiab kev siv khawv koob. Nws cov qauv tsim tshwj xeeb muaj ib lub khob hliav qab ntawm lub cev nrog lub hauv paus dav dav rau ntawm caj dab nqaim, ua kom zoo rau ntau cov ntawv thov kev sim.
Cov flask tus lej conical ua ntau lub hom phiaj. Firstly, nws pab txhawb kev sib xyaw ua ke thiab nplawm cov ntsiab lus vim yog qhov chaw qis, uas pab txo qis chaw uas cov khoom siv tau sib ntxub. Tsis tas li ntawd, dav hauv paus muab kev ruaj khov, txo txoj kev pheej hmoo ntawm lub sijhawm thaum lub sijhawm tswj tau, tshwj xeeb yog thaum siv cov stirrers.
Lub caj dab nqaim ntawm lub conical flask tau txais txiaj ntsig rau ntau yam. Nws tso cai rau cov ntxig yooj yim thiab tshem tawm cov pipettes, spoons, lossis lwm yam twj paj nruag yam tsis muaj cov ntsiab lus, uas yog qhov tseem ceeb hauv ib puag ncig tsis muaj menyuam. Ntxiv mus, nws pab hauv txo cov evaping thiab saib xyuas kev ncaj ncees ntawm kev daws teeb meem lossis cov kev daws teeb meem rhiab. Lub caj dab feem ntau nta daim di ncauj lossis ntug rau kev ruaj ntseg nrog lub chaw nres tsheb lossis lub hau, ntxiv txhim kho lub peev xwm los tswj cov muaj zog thiab tiv thaiv kom tsis txhob muaj kev sib kis.
Cov lus qhia
Cov Ntawv Thov
Tusfungsi conical flaskUa ib qho tseem ceeb ntawm lub luag haujlwm hauv kev tshawb fawb fungi-ntsig txog kev cog qoob loo thiab cov kev sim, kev khaws cia, thiab kev thauj mus los ntawm fungal haiv neeg.
- Fungal Cultivation: Conical flasks yog nquag siv rau kev cog qoob loo, muab ib puag ncig tsim nyog rau lawv txoj kev loj hlob. Lub hauv paus dav tso tawm rau kev sib xyaw ua kom muaj zog thiab aeration, thaum caj dab nqaim txwv tsis pub muaj kev paug.
- Kev Npaj Xov Xwm: Lawv tau siv los npaj thiab khaws cov khoom lag luam fungal kev loj hlob, kom cov khoom noj khoom haus kom zoo rau fungal kev loj hlob yog qhov sib xyaw ua ke thiab faib tawm.
- Kom tsis muaj menyuam thiab aseptic cov txheej txheem: Conical flasks feem ntau tau autoclaved rau sterilize lawv ua ntej siv, ua kom muaj kev tsis muaj zog rau fungal kev sim. Qhov no yog qhov tseem ceeb heev nyob rau hauv kev tiv thaiv kev sib txawv ntawm kev sib kis thiab ua kom pom tseeb.
- Sib xyaw thiab Inoculation: Cov duab ntawm Conical Flask Facilitates yooj yim sib xyaw ntawm cov kab lis kev cai xov xwm thiab inoculation nrog fungal spores lossis mycelium.
- Txhim kho thiab soj ntsuam: Tom qab inoculation, conical flasks yog feem ntau ua kom loj hlob nyob hauv ib puag ncig kom tso cai rau fungal kev loj hlob. Cov khoom siv pob tshab tso cai rau kev soj ntsuam cov qauv ntawm kev loj hlob thiab kev ua tiav yam tsis muaj kev cuam tshuam cov kab lis kev cai.
- Luv luv luv cia: Conical flasks tuaj yeem siv los khaws cov kab lis kev cai fungal ib ntus, tshwj xeeb tshaj yog thaum cov kev sim lossis hloov chaw yuav tsum tau nres.
- Kev mus: Lawv cov qauv thiab kav ntev ua rau lawv haum rau kev thauj cov kab lis kev cai fungal me me lossis txawm rau lwm lub tsev haujlwm rau kev sib koom tes.
Hais txog fungal sau
Fungi yog ib feem tseem ceeb ntawm cov ecosystem, ua si ib qho tseem ceeb ntawm kev txiav txim siab thiab cov khoom noj khoom haus cycling. Cov txheej txheem ntawm fungal kev cais tawm thiab kev cog qoob loo yog cov txheej txheem tseem ceeb hauv kev kawm txog kev cuam tshuam ntawm kev siv ecological. Ntawm no yog ib tug txheej txheem cej luam ntawm cov kauj ruam koom nrog fungal sau qoob rau hauv biochemical kev sim:
Qauv Sau: Thawj kauj ruam yog los sau ib qho qauv los ntawm ib puag ncig uas tau cia siab tias yuav muaj. Qhov no tuaj yeem yog av, cov khoom siv cog ntoo, lossis ua kom cov organic teeb meem. Xyuas kom cov qauv no khaws cov txheej txheem aseptic kom tiv thaiv kom paug.
Npaj cov xov xwm: Ib qho nruab nrab ntawm kev loj hlob nruab nrab yuav tsum npaj rau cov fungi. Feem ntau siv xov xwm suav nrog qos dextrose agar (PDA) thiab yog digouraud Dextrose agar agar (SDA), uas muab cov as-harments rau fungal kev loj hlob. Npaj lub nruab nrab raws li cov chaw tsim khoom cov lus qhia thiab ua kom nws tsis muaj menyuam.
Kom tsis muaj menyuam: Txhawm rau tiv thaiv kev sib kis, cov xov xwm thiab txhua yam cuab yeej siv hauv cov txheej txheem yuav tsum yog sterilized. Qhov no feem ntau ua tiav siv autooclave, uas siv cov kev kub siab thiab ntsuas kub kom tua tej yam tsis muaj kuab paug.
Muab cais nyob ib leeg: Cov qauv yog qhia rau cov me me media. Qhov no tuaj yeem ua tiav los ntawm ntau txoj kev, suav nrog kev ua haujlwm plating ua ke lossis txheej txheem streak txheej txheem, uas pab hauv kev sib cais ntawm cov neeg foob.
Tsim kom lojcation: Inoculated xov xwm tau muab tso rau hauv ib qho tsim kom loj hlob nyob rau hauv cov kev tswj ntawm cov mob kub thiab av noo. Fungi feem ntau nyiam kub ntawm 20-30 degree. Lub sijhawm sib txawv tuaj yeem sib txawv raws li cov tsiaj ntawm cov kab mob fungi tau cog qoob loo, tab sis feem ntau ntawm ob peb hnub rau ntau lub lis piam.
Kev soj ntsuam thiab cim: Tom qab lub sijhawm tsim kom loj hlob, kev loj hlob ntawm fungal ocolies tuaj yeem pom. Cov yam ntxwv xws li Colony morphology, xim, thiab kev ntxhib los mos yog sau tseg. Kev kuaj me me kuj tseem yuav ua rau pom cov qauv microscopic ntawm fungi, xws li cov noob kab mob thiab hypeae.
Kev sib cais: Txhawm rau lim cov fungal cais thiab kom muaj kev tswj hwm lawv lub peev xwm, subculturing yog ua. Qhov no cuam tshuam nrog kev hloov pauv me me ntawm cov fungal colony rau qhov nruab nrab tshiab, tsis huv. Cov kauj ruam no yog qhov tseem ceeb heev rau kev khaws tseg mus ntev thiab kev kawm txog cov fungi.
Kev ceev: Cov hom pwm fungal tuaj yeem khaws cia rau kev siv sijhawm ntev xws li khov-kom qhuav, lossis khaws cia rau hauv glycerol Tshuag ntawm -80 degree. Kev tsim nyog txuag kom ntseeg tau tias cov fungi tuaj yeem kawm thiab siv yav tom ntej kev sim.
Molecular tus xeeb ceem: Rau kev paub tseeb thiab kev faib tawm, cov txheej txheem molecular, cov tshuaj tiv thaiv DNA xws li DNA cov tshuaj tiv thaiv (PCR) tuaj yeem ua haujlwm. Cov tswv yim no muab txoj kev muaj tseeb dua thiab txhim khu kev qha ntawm kev txheeb xyuas cov tsiaj fungi.
Hauv cov kev sim biochemical, fungal sau qoob yog feem ntau siv los kawm cov kev ua yeeb yam metabolic, Enzymatic cov dej num, thiab lwm yam kev ua si ntawm cov khoom ntawm cov khoom lom zem. Nws tseem yog qhov tseem ceeb rau kev rho tawm thiab kev txheeb xyuas ntawm cov kab mob fungi pathogenic, uas tuaj yeem ua rau muaj kab mob hauv tib neeg thiab tsiaj. Kev cais tawm thiab kev cog qoob loo ntawm fungi xav tau kev ua tib zoo soj ntsuam thiab ua tib zoo soj ntsuam kom ntseeg tau tias cov tsiaj uas xav tau loj hlob thiab kawm tau zoo.
Zuag qhia tag nrho, fungal kev cog qoob loo yog cov txheej txheem tseem ceeb hauv Biochemistry uas tso cai rau cov kws tshawb fawb los kawm txog biology, biochemistry, thiab ecology ntawm fungi. Los ntawm kev ua raws li cov theem ntawm cov theem saum toj no, cov kws tshawb nrhiav tuaj yeem ua tiav thiab kawm fungi nyob rau hauv lub chaw kuaj sim ib puag ncig.
Kev Ntsuam Xyuas Kev Ntsuas Cov Duab
Lub hauv paus rau phau ntawv
Cov tshuaj pwm tsis tseem ceeb yog ib qho tseem ceeb ntawm kev tshawb fawb txog kev kho mob, thiab muab cov phiaj xwm tseem ceeb rau kev kho mob thiab kho yeeb tshuaj. Hauv kev sim, cov pwm pob zeb ua ke yog cov kev sim sim, vim yog nws cov duab tshwj xeeb thiab cov khoom siv tau lees paub rau kev kawm du ntawm kev sim. Hauv daim ntawv no, cov hau kev, cov kauj ruam thiab kev ceev faj ntawm fungal tiv thaiv kev sim nrog fungal conical fwj yog qhia hauv kev nthuav dav.
Sim cov ntaub ntawv thiab cov hau kev
Kev sim cov ntaub ntawv
Fungal Conical Lub raj mis: Siv rau kev coj noj coj ua thiab kev ntsuam xyuas kev kuaj mob ntawm fungi, nws cov duab thiab cov khoom siv tsis muaj menyuam thiab tiv thaiv kev sib kis.
Cov tshuaj tiv thaiv kab mob: suav nrog cov tshuaj tiv thaiv kab mob, xws li fluconazole, italaconazole, voriconazole, thiab lwm yam.
Nruab nrab: xws li rpmi 1640 nruab nrab, rau kev loj hlob thiab luam ntawm fungi.
Cov kab mob fungal: cov kab mob fungal: xws li Candida albicans, Candida Tropified, thiab lwm yam.
Xws li cov cuab yeej ua haujlwm aseptic: xws li inoculation rings, cov pas nrig, pipettes, thiab lwm yam, rau kev ua haujlwm aseptic kom tiv thaiv kom tsis txhob muaj paug.
Tas tiittog er incubator: rau kev cog qoob loo ntawm fungi, muab ib qho ntsuas kub haum thiab av noo ib puag ncig.
Kev sim ua
Npaj cov qauv nruab nrab: raws li cov qauv ntawm cov nruab nrab, muaj feem raug tsim nyog, ntxiv rau cov nyiaj uas muaj txiaj ntsig zoo, ua kom sov kom yaj thiab kho tus lej pH rau cov khoom lag luam uas tsim nyog. Cov kab lis kev cai nruab nrab tau muab faib ua cov fungal conical fwj, thiab tom qab ntawd ces sterilized nyob rau hauv ib tug autoclave. Tom qab kom tsis muaj menyuam, txias rau nruab nrab rau qhov ntsuas kub haum thiab teem ib sab.
Inoculation ntawm Fungi: ntawm cov tub rog tsis muaj zog: ntawm cov tub rog me me tau xaiv los ntawm cov pob fungal tau siv lub suab tsis muaj zog thiab inoculated rau hauv qhov nruab nrab tsis huv. Kev saib xyuas yuav tsum tau them rau kev ua haujlwm xws li thaum lub sijhawm ua kom tsis txhob muaj paug.
Kev tsim yeeb tshuaj thiab kev ua haujlwm: Qhov hnyav hnyav: Qhov raug cov tshuaj antifungal tshuaj, yaj nws nrog cov kuab tshuaj uas tsim nyog (xws li DMOPS), thiab dilute nws rau qhov xav tau kev xav tau. Hloov cov yeeb tshuaj tov rau hauv cov leeg kuaj tsis huv thiab teeb ib sab.
Kev ntxiv nrog cov tshuaj thiab kab lis kev cai: kab lis kev cai hauv inoculated fungi yog sib cais nrog cov tshuaj tiv thaiv kev sib txawv, yog li ntawd cov pa taws ntawm cov tshuaj sib txawv. Tom qab ntawd, lub consical lub raj mis tau muab tso rau hauv qhov kub tsis tu ncua, teev ntawm qhov ntsuas kub uas tsim nyog thiab av noo, thiab kev coj noj coj ua. Thaum lub sijhawm kab lis kev cai, kev loj hlob ntawm fungi yuav tsum tau pom tias tsis tu ncua thiab cov ntaub ntawv kaw tseg.
Cov txiaj ntsig kev soj ntsuam thiab kev tsom xam: tom qab kab lis kev cai, lub conical fwj tau tawm mus saib txoj kev loj hlob ntawm fungi. Lub morpi thiab tus naj npawb ntawm fungi tau pom los ntawm lub tshuab tsom thiab cov ntaub ntawv tau sau tseg. Nyob rau tib lub sijhawm, cov ntsuas xws li biomass lossis metabolite cov ntsiab lus ntawm fungi hauv nruab nrab tuaj yeem txiav txim siab ntsuas cov tshuaj tiv thaiv ntawm fungi. Cov ntaub ntawv tau txheeb xyuas tau txheeb xyuas cov teebmeem ntawm cov tshuaj sib txawv ntawm cov tshuaj ntawm kev loj hlob ntawm fungal kev loj hlob thiab kos qhov kev txiav txim siab ntawm kev tawm tsam.
Sim cov txiaj ntsig thiab kev soj ntsuam
Thaum lub sij hawm sim, nws tau pom los ntawm lub tshuab tsom iav pom tias muaj cov tshuaj tiv thaiv kab mob antegi thiab tus lej ntawm cov tub rog maj mam poob qis. Thaum lub concentration ntawm cov tshuaj ncav cuag ib theem, kev loj hlob ntawm fungus yog kiag li suppressed, thiab cov colony tsis tuaj yeem pom. Qhov no qhia tau hais tias cov fungal strain muaj qee cov tshuaj tiv thaiv cov tshuaj raug sim.
Kev txheeb xyuas ntxiv tias qhov kev tawm tsam ntawm cov kab mob fungal sib txawv rau cov tshuaj sib txawv ntawm cov tshuaj tiv thaiv kab mob txawv txawv. Piv txwv li, qee hom kev sib tw yog tiv taus cov fluconazole thiab tsawg tus tiv taus cov nroj me me. Qhov no yuav cuam tshuam txog cov keeb kwm keeb kwm ntawm txoj kev sib cav, ib puag ncig kev loj hlob thiab cov tshuab ntawm kev ua ntawm cov tshuaj.
Kev sib tham
Kev tswj cov mob sim: hauv kev sim, cov haujlwm xws li aseptic yuav tsum tau nruj me ntsis kom tsis txhob muaj kev ua paug ntawm cov txiaj ntsig sim. Nyob rau tib lub sijhawm, nws kuj tseem yuav tsum tau them sai sai rau kev tswj hwm ntawm kev coj noj coj ua, xws li qhov kub thiab lwm yam, kom ntseeg tau tias qhov kev loj hlob ib txwm muaj thiab kev luam ntawm fungi.
Kev xaiv tshuaj yeeb: kev xaiv ntawm kev xaiv cov tshuaj muaj qhov tseem ceeb cuam tshuam rau kev sim ua. Siab dhau lub hauv paus yuav ua rau cov fungus yuav ua rau tsis muaj kev loj hlob, ua rau nws tsis muaj peev xwm ntsuam xyuas nws txoj kev tawm tsam raug nws txoj kev tawm tsam; Yog hais tias lub siab yog tsawg dhau, nws yuav tsis inhibit kev loj hlob ntawm fungus, ua rau muaj kev sim ua tsis tau. Yog li ntawd, nws yog ib qho tsim nyog los ua kev sim ua ntej ua ntej kev sim los txiav txim siab txog kev siv tshuaj kom tsim nyog.
Exploration of drug resistance mechanisms: The generation of fungal resistance may be related to a variety of mechanisms, such as drug efflux pumps, drug target mutations, etc. In the experiment, the mechanism of fungal resistance can be further explored, providing a theoretical basis for clinical treatment and drug development.
Tag
Nws yog txoj hauv kev zoo nqa tawm kev sim fungal tiv taus los ntawm kev siv fungal conical lub raj mis. Cov rhiab ntawm cov neeg sawv cev sib txawv rau cov tshuaj tiv thaiv kab mob txawv tuaj yeem raug ntsuas kom raug yog los ntawm kev tswj cov tshuaj uas tsim nyog siv, thiab soj ntsuam kev loj hlob ntawm cov tshuaj. Cov txiaj ntsig tau pom tias qhov kev tawm tsam ntawm cov tshuaj sib txawv rau cov tshuaj tiv thaiv caj ces txawv, uas yuav cuam tshuam ib puag ncig caj ces, ib puag ncig kev loj hlob, kev ua yeeb yam ntawm cov tshuaj. Yog li ntawd, hauv kev kho mob thiab tshuaj kho yeeb tshuaj thiab tshuaj kho mob tsim nyog rau cov tshuaj tua kab mob thiab cov tshuaj tiv thaiv kev kho mob yuav tsum raug xaiv raws li cov xwm txheej.
Chaw saib xyuas
Nrog rau qhov tob ntawm kev tshawb fawb txog kev kho mob thiab kev txhim kho kev ua haujlwm ntawm kev siv thev naus laus zis, cov hau kev thiab cov tswv yim ntawm fungal kuj kev sim yuav tau hloov tas li. Nyob rau hauv lub neej yav tom ntej, kev tshaj lij thev naus laus zis xws li high-wepose kev ua kom zoo dua qub, muab kev kho mob ntxiv thiab txhim khu kev qha freeoretical kev kho mob thiab kev kho yeeb tshuaj. Nyob rau tib lub sijhawm, cov tshuaj tiv thaiv tshiab thiab cov tswv yim tiv thaiv kev kho mob tuaj yeem tsim los daws cov teeb meem loj hlob ntawm fungal tsis kam.
Tusfungsi conical flask, tseem hu ua Erlenmeyer Flask, yog ib daig tshuab khob iav, nto moo tsim qauv thiab tsim qauv kev ua pov thawj ntawm nws caj dab. Lub caj dab, lub caj dab ntawm lub conical firk ua haujlwm pab ntau lub hom phiaj, suav nrog kev pheej hmoo ntawm cov khoom nchuav thiab txi.
Ib qho ntawm cov tseem ceeb tseem ceeb ua rau nws cov dej xau qhov yog cov snug haum uas tuaj yeem ua tiav nrog lub nres tsheb lossis cov roj hmab bung. Cov kev sib tw sib xyaw ua ke tso cai rau lub foob ruaj ntseg thaum lub chaw nres tsheb tso, uas yog qhov tseem ceeb rau kev tiv thaiv cov ntsiab lus los ntawm kev ua txhaum, tshwj xeeb tshaj yog thaum lub raj mis qaij lossis shaken thaum sim. Cov qauv no tshwj xeeb yog muaj txiaj ntsig thaum daws teeb meem lossis cov tshuaj tsw qab, vim nws ua kom muaj kev nyab xeeb thiab kev nyab xeeb.
Ntxiv mus, cov duab conical cov duab pab hauv kev txo cov roj txaws thiab cov aerosols los ntawm kev khiav tawm ntawm lub khob raj hluav taws xob, txuas ntxiv txhim kho nws cov khoom siv tsis yeem. Thaum pouring kua, cov ceg ntoo uas muaj lub kaum ob tso cai rau kev tswj cov khoom siv, ua kom muaj feem ntawm cov dej ntws dhau lossis ua haujlwm. Zuag qhia tag nrho, lub conical flask lub caj dab caj dab tsim yog qhov tseem ceeb ntawm nws cov haujlwm, muab ob qho kev nyab xeeb thiab ua haujlwm hauv kev kuaj.
Cim npe nrov: Fungsi Conical Flask, Tuam Tshoj Fungsi Conical Breask Manufacturers, Chaw muag khoom, Hoobkas
Tom ntej
Ca Hauv Qab Conical FlaskXa kev nug
