Cool Centrifuge Tshuab
Hauj lwm lawm
Kev tsis
A txias centrifugelub tshuab, tseem hu ua ib qho khoom siv khov, yog lub chaw kuaj mob uas siv zog ua kom cais, tsom xam, emulsions, lossis cov qauv piv txwv ntawm cov ntsuas kub tsawg. Tub yees centrifuges ua ke centrifugal zog thiab cryogenic tswj tshuab. Thaum cov sib tov sib tov yog muab tso rau hauv centrifugal raj thiab pib tig, muaj zog centrifugal zog yog tsim tawm, uas cais cov qauv sib txawv raws li lub ceev sib txawv. Qhov chaw ntom ntom yuav tog rau hauv qab ntawm cov raj hauv qab no, thaum qhov chaw qis qis yuav nyob hauv thaj av loj lossis nruab nrab. Nyob rau tib lub sijhawm, cov ua haujlwm tub yees (feem ntau yog siv lub tshuab compressor rau tub yees) tswj qhov piv txwv tsis pub dhau ntawm cov txheej txheem ntsuas kub ntawm Centrifugation.
Tsawg Qhov Kev Tiv Thaiv Kub
Lub cryogenic tiv thaiv kab ke ntawmtxias centrifugelub tshuabyog ib qho ntawm cov yam ntxwv tseem ceeb uas paub qhov txawv ntawm Centrifuge. Cov txheej txheem no tiv thaiv kev ncaj ncees thiab kev ua si ntawm cov qauv ntsuas kub los ntawm kev tswj hwm qhov chaw ntsuas kub tsawg hauv Centrifugease. Hauv qab no yog cov ncauj lus kom ntxaws ntawm cov kev tiv thaiv cryogenic rau cov tub yees rau hauv tub yees rau cov centrifuges:
Qhov tseem ceeb ntawm kev tiv thaiv kub tsawg
Nyob rau hauv cov txheej txheem centrifugal, qhov kub ntawm centrifugal chamber thiab tus qauv nce vim kev sib txhuam thiab kev ua haujlwm muaj zog tshwm sim los ntawm kev sib hloov nrawm. Rau qhov ntsuas kub-rhiab, xws li cov protein, nucleic acids, cov enzymes thiab lwm cov biomoleces, qhov kev cuam tshuam rau qhov kev cuam tshuam rau qhov tseeb thiab kev ntseeg tau ntawm kev sim ua ntej. Yog li ntawd, cov kev tiv thaiv cryogenic tiv thaiv cov txheej txheem ntawm corderated centrifuge yog ib qho tseem ceeb.
Tus txheej txheem ua hauj lwm ntawm tsawg kev tiv thaiv kub
Lub Cryogenic tiv thaiv lub cev ntawm cov contrifuge centrifuge feem ntau yog suav nrog ntawm tub yees kab tub yees, ntsuas kub thiab txheej txheej txheej.
Cov txheej txheem tub yees yog qhov tseem ceeb ntawm kev tiv thaiv kub tsawg, feem ntau muaj ntawm compressor, condenser, evapator thiab lwm yam.
Lub tshuab ua pa hluav taws xob compresses qhov kub tsawg thiab cov roj siab tsawg dua thiab cov roj siab siab, thiab tom qab ntawd ua kua ua kua tom qab ua kom sov. Raws li cov kua dej ua kua passes dhau los ntawm cov evaporator, nws nqus tau thaum tshav kub los ntawm Centrifugal Chamber thiab cov qauv, yog li txo nws qhov kub.
Cov txheej txheem tub yees tuaj yeem tswj qhov kub thiab txias kom tswj tau qhov kub me me hauv centrifugation txheej txheem, kom pom tseeb tias cov qauv yog centrifuged nyob rau hauv ib pawg tsawg npaum li cas.
Qhov kub tswj hwm cov neeg saib xyuas thiab tswj qhov ntsuas kub ntawm Centrifugal Chamber thiab tus qauv.
Qhov ntsuas kub yog soj ntsuam los ntawm sensor nyob rau lub sijhawm tiag tiag, thiab lub teeb liab tau pub rov qab rau txoj kev tswj hwm.
Cov kev tswj hwm system tau kho cov haujlwm ua haujlwm raws li cov khoom siv ua ntej muaj nuj nqis los tswj hwm qhov chaw kub tsis tu ncua.
Lub centrifugal chamber feem ntau yog nruab nrog ib sab sab hauv rwb thaiv tsev txheej los txo qis thiab kub suab.
Cov thermal rwb thaiv tsev txheej yog feem ntau yog ua kom muaj zog cais cov cua sov thiab tswj qhov tsawg ntawm Centrifugal Chamber thiab tus qauv.
Qhov zoo ntawm qhov chaw tiv thaiv kub tsawg
Tiv thaiv cov qauv kev ncaj ncees thiab kev ua si
Qhov kev tiv thaiv kub tsawg tsawg tuaj yeem tiv thaiv cov qauv ntawm denaturation lossis kev tsis saib xyuas qhov kub thiab txias rau thaum lub sijhawm nruab nrab.
Qhov no yog qhov tshwj xeeb tshaj yog rau cov kev sim uas yuav tsum tau khaws cov qauv kev ncaj ncees, xws li sim hauv kev sim roj ntsha, ntawm kev ua haujlwm biology thiab lwm thaj chaw.
Txhim kho qhov tseeb thiab kev ntseeg tau ntawm kev sim ua
Los ntawm kev tswj kom muaj kev kub ntxhov tsis tu ncua, cov kev tiv thaiv lub ntsej muag cryogenic ua kom muaj kev sib txawv ntawm kev sim.
Qhov no pab txhawm rau txhim kho qhov tseeb thiab kev ntseeg tau ntawm kev sim ua kom muaj txiaj ntsig, thiab muab kev txhawb zog rau kev tshawb fawb.
Nthuav cov kev hloov ntawm daim ntawv thov
Lub tshuab tiv thaiv cryogenic tiv thaiv cov kab ke enables cov controunduge kom lis ntau hom piomolecules, thiab ntau dua.
Qhov no nthuav tawm cov ntawv thov ntau ntawm cov centrifuguges khov thiab ua rau lawv muaj ntau dav siv hauv kev tshawb fawb thiab kev tsim khoom lag luam.
Daim Ntawv Thov Cov Piv Txwv ntawm Kev Tiv Thaiv Kub Kub
Hauv kev kho mob lub chaw kuaj tshuaj, khov canenfuges feem ntau yog siv rau kev sib cais ntawm cov ntshav lossis ntshav, ntshav liab, thiab cov qe ntshav liab. Qhov kev tiv thaiv kub tsawg tuaj yeem tiv thaiv cov ntshav ntawm cov ntshav lossis qhov kub thiab txias rau cov txheej txheem sim ua kom tiav. Nyob rau tib lub sijhawm, nyob rau hauv thaj teb molecular biology, khov cantrifuges tseem dav siv nyob rau hauv kev sib cais molecules xws li DNA, RNA thiab cov protein. Lub tshuab tiv thaiv cryogenic tiv thaiv tuaj yeem tiv thaiv kev ua si thiab cov qauv kev ncaj ncees ntawm cov qauv biomolecules, muab cov qauv kev ntsuas zoo rau kev tshawb fawb tom ntej rau kev sim ua ntej.
Lub ntsiab
Lub cryogenic tiv thaiv kab ke ntawmcqubckhoom tso caimib ceYog ib qho ntawm nws cov yam ntxwv tseem ceeb, uas tiv thaiv kev ncaj ncees thiab kev ua si ntawm qhov ntsuas kub los ntawm kev tswj hwm qhov chaw nyob hauv Centrifuge. Lub kaw lus no tsis yog txhim kho qhov tseeb thiab kev ntseeg tau ntawm kev sim ua ntej, tab sis kuj nthuav tawm cov ntawv thov ntawm cov corderature. Nrog kev nce qib txuas ntxiv ntawm cov thev naus laus zis thiab kev tiv thaiv txuas ntxiv ntawm cov ntawv thov yuav ua lub luag haujlwm khov dua hauv kev tshawb fawb thiab kev tsim khoom lag luam.
Tsawg suab nrov thiab kev tiv thaiv ib puag ncig
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Raws li cov khoom siv uas nquag muaj nyob hauv chav kuaj,cqubckhoom tso caimib cetau nyiam ntau thiab ntau dua los ntawm cov kws tshawb fawb rau nws cov suab nrov qis thiab ib puag ncig kev tiv thaiv. Hauv qab no yog cov ncauj lus kom ntxaws tshuaj xyuas ntawm cov suab nrov thiab ib puag ncig ntawm cov tub yees rau hauv cov centrifuguges:
Tsawg lub suab nrov
Qhov chaw ntawm suab nrov
The noise of frozen centrifuges mainly comes from the friction of its high-speed rotating rotor and internal mechanical components, as well as the airflow noise generated when the centrifuge is running. Cov suab nrov no tsis yog tsuas yog cuam tshuam rau tag nrho ib puag ncig ntawm chav kuaj, tab sis kuj tseem tuaj yeem muaj kev tsis zoo rau lub paj hlwb thiab ua haujlwm ua haujlwm ntawm cov sim.
Tsawg suab nrov tsim
Txhawm rau kom txo cov suab nkauj, cordrifuges feem ntau yog tsim rau:
Rwb thaiv tsev cov ntaub ntawv: suab rwb thaiv tsev rwb thaiv tsev, xws li lub suab rwb thaiv tsev paj rwb thaiv tsev thiab sab hauv ntawm Centrifuge thiab xav txog lub suab nrov.
Precision Raug: Los ntawm precision kev tsim cov txheej txheem, kev sib txhuam thiab kev co ntawm cov tshuab tshuab siv, yog li txo cov suab nrov.
Txhim kho tus qauv: Txhim kho cov qauv sab hauv thiab cov khoom siv tshuab hluav taws xob tau deb, kom cov neeg ua suab nrov nyob deb ntawm tus neeg ua haujlwm sim.
Suab nrov
Feem ntau, cov suab nrov ntawm cov khoom siv nrov nrov ntawm 45 txog 70 lub decibels, nyob ntawm tus qauv, kev coj ua, thiab kev ua haujlwm ntawm cov Centrifuge. Qee qhov kev xaus ntawm cov khoom siv khov kho tshiab, xws li Eppendorf 5427r, feem ntau yog cov suab nrov txo cov thev naus laus zis, uas yog qhov zoo tagnrho rau cov suab nrov-rhiab.
Kev tiv thaiv ib puag ncig kev tiv thaiv
Ib puag ncig phooj ywg tub yees
Hauv cov tub yees centrifuges xav tau kev siv cov tub yees hauv tub yees txheej txheem. Tsu cov tub yees ua paug rau ib puag ncig, xws li Freon. Txhawm rau tiv thaiv ib puag ncig, niaj hnub ua tub yees cov centrifuguges feem ntau siv cov tub yees zoo ib puag ncig xws li propane (R290). Cov tub yees no yog ntuj, tsis muaj tshuaj lom, tsis muaj peev xwm, thiab tsis muaj kev puas tsuaj rau cov roj ozone txheej, pab kom txo cov roj tsev cog khoom pa tawm.
Zog txuag tsim
Txhawm rau kom txo cov hluav taws xob thiab txo cov pa roj carbon monissions, cov tub yees niaj hnub centrifuges feem ntau siv lub zog tsim muaj zog:
Lub cev muaj zog ua haujlwm hnyav: Siv kev ua haujlwm siab thiab lub zog txuag lub cev muaj zog kom txo qis kev siv zog.
Cov kev tswj hwm ntse: los ntawm cov hauv paus hniav ntse, lub xeev ua haujlwm ntawm lub tub yees txheej txheem kaw tau hloov kho raws li qhov xav tau tiag tiag kom tsis txhob siv fais fab tsis tsim nyog.
Standby hom: qee qhov Centrifuges muaj Eco tsis siv neeg sawv, uas cia li kaw cia thaum tsis siv txuag lub zog thiab txuas ntxiv cov cuab yeej siv lub neej.
Cov Khoom Siv Uas Siv Rov Ua Dua
Cov tub yees niaj hnub rau centrifuges nce siv cov khoom siv ua dua tshiab hauv kev tsim khoom. Cov ntaub ntawv no tuaj yeem rov ua haujlwm tom qab cov khoom siv tau muab pov tseg, txo qis kev noj ntawm cov khoom muaj kuab paug ntuj thiab ib puag ncig muaj kuab paug.
Kev muaj ntawv pov thawj ib puag ncig
Qee lub npe siab kawg ntawm cov tub yees rau hauv tub yees tau muaj ntawv pov thawj rau kev tiv thaiv ib puag ncig, xws li cov eppendorf 5427r uas tau ntawv pov thawj los ntawm kuv tus ACL tshiab. Cov ntawv pov thawj no qhia tau tias cov cuab yeej ua tau raws li tus txheej txheem siab ntawm kev tiv thaiv ib puag ncig.
Kev Ntsuas Xyuas
Tsawg lub suab thiab kev tiv thaiv ib puag ncig yog ib qho tseem ceeb ntawm cov yam ntxwv niaj hnubcqubckhoom tso caimAchinesCov. Lub suab nrov tsawg dua tuaj yeem muab cov chaw ua haujlwm ntsiag to thiab xis nyob, uas pab txhim kho kev ua haujlwm thiab kev mob hlwb ntawm cov sim. Ib puag ncig cov yam ntxwv ib puag ncig pab kom txo qhov cuam tshuam rau ib puag ncig thiab txhawb nqa txoj kev loj hlob ntawm kev lag luam khoom siv. Thaum xaiv lub colentreed khov, cov kws tshawb nrhiav yuav tsum xav txog cov suab nrov thiab ib puag ncig cov khoom siv, thiab xaiv cov khoom uas tau raws li lawv cov kev xav tau.
Tsis tas li ntawd, nrog kev nce qib txuas ntxiv ntawm cov thev naus laus zis thiab kev nthuav dav txuas ntxiv, lub neej yav tom ntej ntawm cov suab nrov khov hauv cov suab nrov tsawg thiab ib puag ncig. Piv txwv li, ntxiv txo cov khoom suab nrov thiab kev siv zog los ntawm kev siv lub suab nrov ntau thev naus laus zis thiab cov ntaub ntawv ib puag ncig; Los ntawm cov tswv yim tswj hwm ntse, tsis siv neeg ua haujlwm txhim kho ntawm cov khoom siv tau pom tau. Cov kev tawm tsam no yuav muab cov kws tshawb fawb nrog cov khoom siv kuaj thiab ib puag ncig ntawm kev txhim kho ntawm kev tshawb fawb ntxiv.
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