Caw Kaw Lus Flash Sab Daim Chromatography
2.Chromatogratographic Kem (Hloov Hom)
3.Chromatographic Tag (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
Caw Kaw Lus Flash Sab Daim ChromatographyYog kev sib cais sib cais sib cais, tshwj xeeb yog tsim nyog rau kev sib cais sai thiab ua kom huv ntawm cov mixatures ceev. Nws cov ntsiab cai yooj yim zoo sib xws rau kem Chromatograph thiab nyias-txheej Chromatograph, ob qho tib si yog nyob ntawm qhov sib txawv ntawm cov theem nyob sib txawv ntawm cov theem ruaj khov ntawm theem ntsuas kev sib cais. Txawm li cas los xij, tsis siv neeg ceev ceev pide chratography txhim kho kev sib cais txuag thiab nrawm los ntawm kev siv cov khoom siv tshuab thiab cua compressed kom thawb cov kuab tshuaj.
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Chaw ua uas tsis tau haus
Cov hauv paus ntsiab lus ntawm physics thiab kev ua haujlwm ntawm cov txheej txheem
1. Cov ntsiab cai ntawm Physics
(1) Kev faib tawm coefficient: kev faib tawm coefficient ntawm theem nyob nruab nrab ntawm thaj chaw nyob nruab nrab thiab mobile theem yog qhov tseem ceeb txiav txim siab nws tus nqi sib cais. Qhov loj dua faib cov coefficient, ntev dua lub sijhawm khaws cia ntawm cov tshuaj ntawm cov theem ruaj khov; Ntawm qhov tsis sib xws, qhov me me faib mis coefficient, nrawm dua cov khoom txav nrog lub mobile theem.
(2) Adsorption muaj peev xwm: tus adsorption muaj peev xwm ntawm cov theem ruaj khov (xws li Silica Gel) ntawm cov tshuaj kuj tseem tuaj yeem cuam tshuam rau kev sib cais. Cov tshuaj nrog muaj peev xwm ua tiav muaj peev xwm muaj lub sijhawm nyob ntev dua nyob rau theem chaw ruaj khov, thaum cov tshuaj nrog muaj peev xwm tshaj tawm muaj peev xwm ntau dua nrog cov theem mobile.
(3) solubility: lub solubility ntawm cov tshuaj hauv cov theem mobile kuj cuam tshuam rau nws tus nqi sib cais tseem cuam tshuam. Cov tshuaj nrog cov tshuaj lom solubility siab dua nrog cov mobile theem, thaum cov tshuaj nrog solubility tsis tshua muaj nyob ntawm theem qis dua.
2. Cov txheej txheem ua haujlwm
(1) Qauv npaj: ua ntej, nws yog qhov tsim nyog los txiav txim siab lub dryness theem ntawm cov sib tov kom sib cais kom sib cais thiab ntsuas nws. Tom qab ntawd, raws li cov txiaj ntsig ntawm nyias-txheej Chromatograph, xaiv cov kuab tshuaj uas tsim nyog los khaws cov nqi RF tsis pub dhau ib qho (feem ntau}. 2 ~ {4. 3). Rau cov mixatures, gradient Elient yuav tsim nyog.
(2) Qauv Kem Chaw thau khoom: Nyob ntawm cov khoom ntawm cov qauv, cov qauv kev daws teeb meem, lossis silica gel adsorption txoj kev tuaj yeem xaiv tau los thauj cov qauv rau hauv lub Colum. Cov qauv qauv qauv yog qhov tsim nyog rau cov tshuaj pleev uas tsis muaj kuab txhuas yog qhov tsim nyog rau cov qauv ua kua thiab cov qauv ua kua yog qhov tsim nyog rau qee yam kua thiab txhua yam khoom siv.
(3) Filling Silicone Tom qab ntawd, sau cov fume hood nrog ib qho kev sib cais, ua kom cov colum yog ntsug thiab tsis txhob poob ntawm silicone gel. Ntxiv xuab zeb ua txheej txheej tiv thaiv nyob rau sab saum toj ntawm silicone kom tiv thaiv kev puas tsuaj rau lub silicone txheej.
(4) Kev Khom Nyem thiab Sau: Ntxiv Eluent rau Kev Sib cais Colum los pib cov txheej txheem kom tsawg. Ua kom muaj tus nqi ntws uas tsim nyog thiab tsis tu ncua hloov cov hlua sib sau. Lub caij no, nyias cov txheej khij cov lej khij cov cim tau los taug qab txoj kev sib cais los xyuas kom meej ntawm cov qauv dawb.
(5) Gradient elient: Rau cov sib xyaw ua ke sib xyaw, gradient elient yuav tsim nyog. Maj mam hloov pauv lub polarity ntawm eluent rau elute tebchaw ntawm txawv polarities. Thaum lub sijhawm kawm tiav qib siab, nws yog qhov yuav tsum maj mam nce cov polarity ntawm cov kuab tshuaj kom tsis txhob silica gel splitting thiab txo qis hauv kev sib cais ua haujlwm.
Daim Ntawv Thov Cheeb Tsam
Caw Kaw Lus Flash Sab Daim ChromatographyCov thev naus laus zis tau siv dav hauv ntau daim teb vim nws qhov kev ua tau zoo, nrawm, thiab yooj yim ntawm kev siv tshuab. Nov yog qee qhov ntawm cov chaw thov tseem ceeb:
(1) cov khoom lag luam zoo nkauj rho tawm thiab sib cais: Tsis siv neeg cov thev naus laus zis tuaj yeem siv los rho tawm thiab nyias muaj kev sib txuas ntawm cov khoom ntuj. Cov tebchaw no muaj cov kev cia siab ua ntej hauv kev ua yeeb yam xws li kev tsim tshuaj yeeb, kev lag luam khoom noj, thiab tshuaj pleev ib ce.
(2) Khoom siv yeeb tshuaj thiab kev ua kom huv: nyob rau hauv cov txheej txheem ntawm cov khoom siv yeeb tshuaj, tsis siv neeg ceev cov khoom siv hluav taws xob thiab cov kev tshem tawm ntawm impurities. Qhov no yuav pab txhim kho cov tshuaj huv thiab cov tshuaj zoo, yog li tau raws li cov cai tswj cov tshuaj uas muaj tshuaj lom neeg.
(3) Kev Ntsuam Xyuas Ib Cheeb Tsam muaj kev soj ntsuam ib puag ncig yuav siv tau rau kev tsom xam thiab kuaj pom ntawm cov neeg muaj kuab paug hauv cov kuab paug hauv ib puag ncig. Los ntawm kev xaiv tsim nyog nyob ruaj ruaj thiab mobile theem mob, kev sib cais ntawm cov kab mob ib puag ncig tuaj yeem ua tiav, muab kev tshawb fawb rau ib puag ncig kev pab pawg kom tswj tau ib puag ncig.
(4) Kev kuaj zaub mov rau kev nyab xeeb Qhov no pab kom muaj kev nyab xeeb thiab cov khoom noj zoo, thiab kev tiv thaiv kev noj qab haus huv ntawm cov neeg siv khoom.
(5) Kev Tshawb Fawb Biochemistry:
Hauv kev tshawb fawb Biochemistry, tsis siv neeg sai sai cov tshuaj tua kab mob thiab cov khoom siv roj ntsha ua si hauv cov kev tshawb fawb lub neej, thiab kuaj mob ntawm tus kab mob, thiab kho kab mob.
Lub cev tshwm sim thiab yam cuam tshuam nrog
Caw Kaw Lus Flash Sab Daim ChromatographyThev naus laus zis tsis muaj cov khoom siv lub cev, nws cuam tshuam nrog ntau yam kev ua haujlwm lub cev thiab cov yam tseem ceeb rau kev nkag siab thiab ua kom zoo dua qub thev naus laus zis.
1. Lub cev cov khoom ntawm mobile theem
(1) Qhov kev xaiv ntawm cov kuab tshuaj:
Lub zog ntawm lub cev ntawm cov kuab tshuaj, xws li polarity, viscosity, ceev, muaj qhov cuam tshuam tseem ceeb rau kev sib cais. Xaiv cov kuab tshuaj uas tsim nyog tuaj yeem paub meej tias cov tshuaj yuav tsum sib cais muaj qhov tsim nyog faib cov kauj vab uas tsim nyog nyob rau theem thiab cov mobile theem.
(2) tswj hwm ntawm tus nqi txaus:
Tus nqi txaus yog qhov tseem ceeb cuam tshuam rau kev sib cais nrawm. Tus nqi txo qis yuav ua rau lub sijhawm sib cais, tab sis nws kuj tseem yuav ua rau muaj kev txo qis hauv kev sib cais. Yog li ntawd, nws yog qhov tsim nyog los kho tus nqi txaus raws li qhov xwm txheej tiag tiag kom ua tiav qhov kev sib cais zoo tshaj plaws.
2. Lub zog ntawm cov theem nyob ruaj khov
(1) Cov khoom ntawm Silicone:
Silicone yog ib qho ntawm cov khoom siv uas feem ntau siv nyob ruaj ruaj. Lub zog lub cev xws li qhov loj me me, Porosity, thiab thaj chaw tshwj xeeb tuaj yeem cuam tshuam rau kev sib cais. Silica gel nrog me me particle loj muaj qhov loj me deg thaj tsam thiab porosity, uas tuaj yeem muab kev sib cais zoo dua; Txawm li cas los xij, cov me me uas yog cov me me dhau kuj yuav ua rau muaj kev thaiv plab thiab txo qis hauv kev sib cais ceev.
(2) kev ruaj khov ntawm cov theem ruaj khov:
Qhov ruaj khov ntawm cov theem ruaj khov yog qhov tseem ceeb rau kev ua haujlwm ntev thiab kev sim ua dua. Theem ruaj khov ua haujlwm tuaj yeem ua kom muaj qhov tseeb thiab kev ntseeg tau ntawm kev sib cais tau.
3. Lub cev cov qauv ntawm cov kab sib cais
(1) Qhov loj thiab cov duab ntawm cov kem:
Qhov loj thiab cov duab ntawm lub colum yuav cuam tshuam rau kev sib cais ua haujlwm thiab ntws tus nqi. Tus luv thum colum yuav ua rau lub sijhawm sib cais, tab sis nws kuj tseem yuav ua rau txo qis hauv kev sib cais; Cov zaub mov ntev dua tuaj yeem muab kev sib cais zoo dua, tab sis lub sijhawm sib cais tseem yuav ua ntev ntev ntev. Cov duab ntawm lub Colum kuj cuam tshuam txog kev sib cais ua tau, thiab cylinder colums feem ntau yog xaiv los xyuas kom meej cov ntu ntawm lub xov xwm mobile.
(2) Cov tawv nqaij ntawm cov thawv:
Cov nruj ntawm cov ntim yuav cuam tshuam rau tus nqi txaus thiab sib cais ua tiav ntawm cov mobile theem. Overly nruj ntim tej zaum yuav ua rau qhov txo qis hauv kev txo qis thiab sib cais efficiency; Cov ntim xoob xoob yuav ua rau colum thaiv thiab qhov sib cais tsis tiav.
4. Tus cawv ntawm kub thiab lub siab
(1) Tswj Kub:
Qhov kub yog ib qho tseem ceeb rau kev cuam tshuam rau kev sib cais. Tsim nyog qhov ntsuas kub tuaj yeem ua kom pom tseeb qhov tsis muaj zog ntawm cov kuab tshuaj thiab solubility ntawm cov tshuaj yeeb dej caw, yog li kev txhim kho kev sib cais ua ke. Qhov kub thiab txias ntau dhau yuav ua rau cov ntsuas kev kho mob evaporation sai thiab quav yeeb tshuaj; Txawm li cas los xij, kev kub dhau ntau dhau yuav ua rau txo qis hauv cov solidification thiab sib cais tus nqi.
(2) kev tswj siab:
Hauv tsis siv neeg ceev nras tsaj Yog li ntawd, kev tswj siab yog qhov tseem ceeb heev rau kev ua kom muaj kev ruaj khov ntawm cov nqi txaus thiab qhov tseeb ntawm kev sib cais ua haujlwm.
Daim ntawv thov thiab ua kom zoo ntawm lub cev tshwm sim
(1) diffusion thiab convection:
Nyob rau hauv kev sib cais cov txheej txheem, diffusion thiab convey yog ob qho kev tshwm sim tseem ceeb ntawm lub cev. Diffusion hais txog cov txheej txheem hloov chaw los ntawm cov cheeb tsam siab ntawm cov cheeb tsam kom tsawg ntawm cov kev ua kom muaj peev xwm; Convention yog hais txog kev hloov pauv ntawm qhov teeb meem tshwm sim los ntawm cov kua suab. Nyob rau hauv tsis siv neeg ceev cov paib colum chromatograhy, diffusion thiab convection ua haujlwm ua ke cuam tshuam rau kev sib cais nrawm thiab ua haujlwm tau zoo. Yog li ntawd, nws yog qhov tsim nyog los txhim kho kev sib raug zoo thiab kev xa cov nrawm los ntawm kev ua haujlwm zoo thiab ua tiav kev sib cais zoo dua.
(2) chromatograph nyhuv:
Chratograph tau hais txog cov txheej txheem ntawm ntau cov adsorption thiab desorption ntawm cov tshuaj ntawm cov theem nyob ruaj khov ntawm theem thiab cov mobile theem. Cov nyhuv no tuaj yeem ua kom lub sijhawm ua haujlwm nyob hauv lub colum, yog li kev txhim kho kev sib cais ua haujlwm. Txawm li cas los xij, ntau dhau cov chomratographic kuj tseem yuav ua rau txo qis hauv kev sib cais nrawm thiab colum thaiv. Yog li ntawd,Caw Kaw Lus Flash Sab Daim Chromatographyyog qhov tsim nyog kom sib npaug ntawm cov kev sib raug zoo ntawm chromatograph cov nyhuv thiab sib cais nrawm thaum lub sijhawm ua haujlwm kom ua tiav qhov sib cais zoo tshaj plaws.
Cim npe nrov: Caw Xov Tooj Flash Sab Chromatography, Tuam Tshoj Automated Flash Sab Chromatograph Cov Chaw Lag Luam Chromatography, Lwm tus neeg, Hoobkas
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