Muaj cov chromatography Sab

IbinatustialcHROMATGODEctus hlisyog ib qho cuab yeej siv tshuaj ntsuam uas cais cov sib xyaw thiab kev lag luam ib puag ncig ntawm cov ntsuas hluav taws xob, kev siv hluav taws xob ntawm cov khoom sib cais ntawm cov khoom sib cais thiab kev sib cais ntawm cov sib txawv ntawm cov khoom sib txawv. ib kem feem ntau muaj Ib qho chaw nyob ruaj khov thiab cov lej mobile. Cov qauv ntawm cov sib nrug

Kev sib cais cov nyhuv ntawm kab ntawv xaiv nyob ntawm theem nyob ruaj ruaj, thiab cov pa roj carbon monoxide (}) (SCX), phenyl), phenyl), amino kem (a) Mino / NN4), CyNO / Niturile, thiab lwm yam}

Muaj cov chromatography tag muaj ntau cov kev siv hauv ib tus naj npawb ntawm cov teb, suav nrog:

Biopharraceutical:Cov Chromatographic kem yog lub ntsiab tseem ceeb ntawm kev sib cais thiab siv rau kev ua vaj tse thiab cov khoom lag luam siab lom {}}
Zaub Mov Kev Nyab Xeeb:Siv los xyuas cov tshuaj tiv thaiv cov tshuaj xws li cov khoom ntxiv thiab cov seem hauv cov zaub mov kom ntseeg tau kev nyab xeeb zaub mov {}}
Saib Xyuas:Kev sib cais thiab tsom xam ntawm cov pa paug nyob hauv ib puag ncig thiab kev txheeb xyuas ntawm ib puag ncig zoo .
Petrochemical:Siv rau kev coj dawb huv tau kom pom kev ntawm cov roj av, kev tsom xam ntawm kev tsis sib raug mob, thiab lwm yam {}}

Hauv ntau daim teb xws li kev soj ntsuam ib puag ncig yog qhov kev ua haujlwm ntawm cov khoom siv thiab kev ua tau zoo ntawm cov chaw ua haujlwm yog qhov kev ua tau zoo ntawm cov hom thiab cov ntsiab lus tseem ceeb ntawm kev siv cov ntsiab lus ntawm kev lag luam chromatographic txhua, kom muab cov lus siv muaj txiaj ntsig rau cov kws tshawb nrhiav hauv cov teb ntsig txog.

Hom Packing

Kev xaiv ntawm kab kem yog dav, thiab raws li cov ntaub ntawv yooj yim, nws tuaj yeem faib ua cov khoom siv roj ntsha thiab cov tshuaj lom neeg thiab cov tshuaj lom neeg.

Inorganic muab tub lim

Inorganic fillers paub txog lawv cov tshuab ntsuas siab ruaj khov, thiab yog tsim nyog rau kev sib cais nrog cov pa roj hmab thiab cov pa roj carbon monoxide

Silica gel yog ib qho ntawm cov neeg siv hluav taws xob hauv cov roj ntsha li cas xws li cov pab pawg chromatography, thiab rov qab ua haujlwm hauv chav nyob ruaj khov Cov chromatography, silica gel nto sib luag muaj cov pab pawg ua haujlwm zoo, xws li C18, C8, C8, C8, C8,

Alumina kuj yog ib hom kev muab tub lim, thiab nws cov lus yog qhov tsim nyog rau lub sijhawm ntev nrog rau kev ua haujlwm ntev rau hauv 12,}

Gratitized carbon yog ib hom tshiab ntawm inorganic muab tub lim uas tsis muaj lwm yam kev sib tw ntawm cov pa roj carbon monominal siv nyob rau hauv txhua pH thiab kub .

Zacconia muab tub rog yog ib qho ntawm cov tub rog tshiab inorganic kawm nyob rau hauv xyoo tas los yog polymer} nws yog ib daim ntawv thov dav dav {}

 

Organic Fillers

Cov organic fillers tsuas yog suav nrog polymer fillers, xws li polystyriat-{} {} {} {} {} {} {} {} {} Kuv muaj zog rau kev sib cais ntawm macromolecules xws li Proteins . Txuas ntxiv, cov organic fitters muaj cov tshuaj thiab haum rau ntau qhov kev sib cais ntawm kev sib cais.

 

Kev Pab Cuam Sib Tw Roj ntsha

COMALS BIBS yog tsim nyog rau kev tsom xam cov roj ntsha, xws li cov protein ntau lossis cov protein) biomolecules .

Cov kev xaiv ntawm cov ntawv ntim khoom haum yog tus yuam sij kom ntseeg tau cov nyhuv ntawm chromatography. Thaum xaiv cov fillers, cov hauv qab no yuav tsum tau txiav txim siab:

 Sib cais hom

Raws li cov khoom ntawm analytes, qhov tsim nyog chrataphic hom yog xaiv cov chromatography (romic),} {{1} {1} sib cais hom muaj txawv cov kev xav tau rau cov lim dej, xws li ib ntu chromatography feem ntau yog cov tub sau cov roj ntsha liners raws sillicaphy soms puv polar {}}

 THIBE TXAUS PASE THIAB TXAWB THEEM

Cov kev xaiv ntawm theem ruaj khov nyob ntawm cov polarity, molecular loj thiab cov qauv ntawm lub annalte {} Cov tub sau qoob loo tuaj yeem raug xaiv, thiab pob zeb hydrotshilic; Rau cov khoom tsis muaj-polar, cov tub rog uas tsis xaiv, ib ntu ntawm lub sijhawm, hauv kev sib cais ua kom muaj qhov sib cais zoo li cas los yuav tsum qis dua li cov theem nyob ruaj khov; Hauv qeb Reversed chromatography, kev polarity ntawm mobile theem yog lub zog loj dua.

 Particle Loj Thiab Aperture

Lub particle loj ntawm cov muab tub lim rau sab nrauv cuam tshuam rau kem eagiticiency thiab coj mus rau seem sib xyaw ua ke, tab sis tuaj yeem ua rau nce rov qab siab; Cov ntxhib cov khoom siv tau txais txiaj ntsig coj mus ua qauv, tab sis tus pore sib cais yuav tsum tau ua kom muaj txiaj ntsig zoo dua li, kev xaiv Thaum cov tub rog me me uas muaj txiaj ntsig zoo los txhim kho cov kev tuav pov hwm ntawm cov molecular sib xyaw me me.

 Tshuaj Muaj Peev Xwm

Cov tub yees siv xaiv yuav tsum yog cov tshuaj ruaj khov raws li cov qauv lossis kev ntseeg tau, nws yog qhov tsim nyog los xaiv cov tub lim dej ua ke nrog kev sib cais nrog cov tshuaj ruaj khov rau kev ruaj khov.

 Hom thiab Nqi

Cov tub rog sab sib txawv yuav sib txawv hauv kev ua tau zoo, tab sis kuj yuav tsum tau txiav txim siab tus nqi them rau cov xwm txheej thiab xaiv cov khoom lag luam thiab xaiv cov khoom siv tau nqi.

► Ua kom huv ntawm cov tshuaj pleev roj monoclonal (Mabs) hauv Biophartuticals

Qhov nyob nram qab

A global biotech company aimed to scale up the purification of a therapeutic monoclonal antibody (mAb) from a 50 L pilot batch to a 1,000 L commercial batch. The challenge was to maintain >99% kev coj dawb huv thaum tsis muaj peev xwm txo cov protein thiab tswv yim ntawm tes (HCP) paug.

Txheej txheem

Sab Tsim:

Ib tug 300 mm sab hauv lub taub (ID) × 500 mm steein afinity resin (Mabselect Nco ntsoov, cytiva).

Qhov siab tshaj plaws kev khiav hauj lwm siab: 3 bar .

Txheej txheem tsis:

Kev kho tsis zoo: 20 hli sodium phosphate, pH 7.4.

Kev xaiv tsa Buyer: 0.1 m citric acid, pH 3.0.

Flow tus nqi: 150 ml / min (linear velocity: 100 cm / h) .

Kev tso tshuab ua:

Kev sib xyaw ua ke siv cov ntsuas hluav taws xob rau PH, conductivity, thiab UV Nrhiav tau (280 nm).

Caw kaw lus tu-hauv-chaw (CIP) nrog 0 {. 5 m naoh.

Qho kawg

Tos: 85% (1,020 g ntawm Mab los ntawm 1,200 g crude pub) .

Purity: 99 . 5% (HPLC Kev Ntsuam Xyuas).

Cov ntsiab lus yuam kev:<1% (size-exclusion chromatography, SEC).

HCP Txo: Los ntawm 50, 000 ppm rau<5 ppm (ELISA assay).

Tseem Ceeb Takaways

Scalability: cov kab ntawv L / d piv (ntev-rau-txoj kab ntawm 1}}}}} zoo ib yam kev ua tau zoo nyob rau hauv cov nplai.

Resin Life: cov protein ib tug resin muaj sia nyob 100 mus txog nrog CIP, txo tus nqi ntawm 30% .

Automation: Kev saib xyuas tiag tiag tau txo cov kev cuam tshuam los ntawm kev cuam tshuam los ntawm 70%, kev txhim kho txheej txheem kev ntseeg tau {}}

Hauv cov ntawv thov siv, tom qab xaiv cov ntawv ntim uas tsim nyog, cov ntsiab lus hauv qab no yuav tsum tau them nyiaj rau kom ntseeg tau tias cov nyhuv chromatographic sib cais:

 Ntawv pov thawj Nyeem cov phau ntawv qhia cov ntawv qhia

Ua ntej siv cov kem tshiab, koj yuav tsum ua tib zoo nyeem nws cov lus qhia kom nkag siab cov chaw muab tub lim, kev siv kev pabcuam kom raug thiab txuas ntxiv nws lub neej lub neej.

 Siv ib kem zoo

Nco ntsoov tias cov kab ntawv tau zoo, tsis muaj npuas, tsis muaj qhov tawg thiab lwm yam ntawm cov chromatographic cais cov nyhuv.

 Txo kev ntxhov siab kev hloov pauv

Nyob rau hauv cov txheej txheem ntawm Chromatographic sib cais, siab tsim nyog yuav tsum tsis txhob muaj cov tshuab thiab cua sov {} {0} .

 Siv cov ntawv tiv thaiv thiab cov lim hauv kab

Txhawm rau tiv thaiv kem rau ntawm kev sib kis thiab puas, cov khoom lim dej thiab cov kab hauv kab thiab cov kab ke khaws cia hauv cov qauv thiab mobile theem.

 Ntxuav kem ntau zaus nrog cov kuab hnyav

Cov flushing tsis tu ncua ntawm kem nrog cov kuab tshuaj uas muaj zog thiab cov khoom tsis huv uas tseem ceeb tshaj plaws rau saum npoo ntawm tus muab tub lim thiab tswj hwm nws cov kev sib cais zoo .

 Full full cov qauv thiab mobile theem

Ua ntej txhaj cov qauv thiab mobile theem rau hauv kem, nws yuav tsum tau tag nrho cov lus phem rau kev tshem tawm cov khoom tsis sib cais thiab txo kev sib cais.

 Tswj Kab Kub

Qhov ntsuas kub ntawm cov ntawv muaj qhov cuam tshuam tseem ceeb ntawm nws sib cais ua ke nrog cov kab mobile, ntsuas kub}}}

Cim npe nrov: Muaj cov chromatography Sab, Tuam Tshoj muaj chromatography Sem Manufacturers, lwm tus neeg, lub Hoobkas