Gel Cov Lus Pom Cov Chromatography Sab
2.Chromatogratographic Kem (Hloov Hom)
3.Chromatographic Tag (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
TusGel Cov Lus Pom Cov Chromatography SabPuas yog kua tshuaj chromatography cov txheej txheem rau kev sib cais thiab tsom xam ntawm cov molecular cov molecular polymer. Cov tub ntxhais tivthaiv yog ib kem nrog cov ntim ntxig, uas tau sib cais los ntawm kev ntsuas cov txheeb ze ntawm cov qauv hauv lub xov xwm mobile. Nws cov ntsiab cai yooj yim yog lub ntxig gel nrog qee yam void loj li kev sib cais nruab nrab. Thaum sib xyaw ua ke muaj cov hnyav hnyav molecular tau ntxiv rau hauv kem, cov tshuaj no tau hloov nrog lub tuam txhab mobile. Vim tias muaj cov hws sib txawv hauv cov gel, cov tshuaj nrog cov hnyav molecular yuav thaiv tau rau cov qib sib txawv thaum lawv dhau ntawm cov kab. MacRomolecular tshuaj tsis tuaj yeem sau cov khoom noj gel, tsuas tuaj yeem txav tawm sai raws qhov sib txawv ntawm cov khoom gel, yog li lawv tab tom muaj kev vam meej thaum ntxov. Molecules me me tuaj yeem nkag mus rau hauv cov gel me me, thaiv, txav maj mam, thiab eluate tom qab. Nruab nrab-qhov loj me molecules tuaj yeem nkag mus rau cov gel cov khoom, thiab cov nyhuv rov qab nruab nrab ntawm lub sijhawm loj thiab me me kuj nyob nruab nrab ntawm ob. Los ntawm kev siv qhov kev txav no sib txawv, cov tshuaj nrog hnyav molecular hnyav tuaj yeem sib cais.gel chaw tshawb nrhiav tau sib cais, kev tshawb fawb tshuaj thiab lwm thaj teb. Nws tuaj yeem siv los cais thiab ntxuav cov protein, enzymes, nucleic acids thiab lwm yam kev faib cov roj ntsha molecules ntawm polymers.
Chaw ua uas tsis tau haus
Kev faib tawm thiab cov ntawm daim ntawv thov
Gel Cov Lus Pom Cov Chromatography Sab, raws li qhov ua tau zoo heev thiab dav siv nyob rau hauv biochemistry, molecular biology thiab lwm lub tsev sib cais ua ke, raws li qhov loj me ntawm cov qauv molecuel) sib cais. Hauv qab no yog cov ncauj lus kom ntxaws ntawm kev faib tawm thiab cov kev siv daim ntawv thov ntawm cov gel pentration txhua.
Kev faib tawm gel favtration chromatogy txhua
Qhov kev faib tawm ntawm Gel cov lus pom tseeb yog tsuas yog raws cov gel cov khoom siv thiab cov xov xwm sib cais. Feem ntau, cov kua nplaum tuaj yeem muab faib ua ob hom kev txiav txim siab ntawm cov tshuaj tua kab mob hauv cov organic (GPC) siv cov kev daws teeb meem zoo sib xws Kev sib xws, cov hauv qab no tsom rau GFC thiab nws cov kev faib tawm feem ntau). Hauv kev siv cov kev thov, txawm li cas los xij, kev faib tawm tuaj yeem paub ntau ntxiv vim muaj kev sib txawv thiab tshwj xeeb ntawm hom kev faib tawm ntawm hom yog tseem muaj lus qhia.
Aqueous Gel rictration chromatography (GFC)
Nta: Feem ntau yog siv dej li cov mobile theem, nws yog qhov tsim nyog rau kev cais cov dej-solubble roj macromolecules, xws li cov protein, peptides, nucleic acids, thiab lwm yam.
Gel hom: feem ntau siv gels muaj xws li Dextran gels, agar-agar gels, agar muaj zog thiab tuaj yeem tswj tau zoo thiab sib cais ua rau muaj aqueous.
Organic Hnyav Gel chromatography (Txawm hais tias tsis ua nruj me ntsis cais ua GFC, tab sis tau hais los ua kom pom cov breen ntawm gel chromatography)
Txawm hais tias nqe lus no tsom rau GFC, nws tsim nyog sau cia uas muaj cov lus qhia zoo xws li cov roj av uas muaj kuab yooj yim, thiab cov khoom siv hluav taws xob uas muaj kev yaig los ntawm cov kuab tshuaj organic los ntawm cov kuab tshuaj organic.
Cov kev sib tw ntawm daim ntawv thov ntawm Gel lim cov cim chromatogromized
Gel Cov lus pom zoo muaj ntau cov ntawv thov, xws li los ntawm kev tshawb fawb yooj yim rau kev lag luam khoom lag luam. Nov yog qee qhov ntawm cov chaw thov tseem ceeb:
Biochemistry
Protein purification: cov Gel cov lus teb rau sab yog ib qho ntawm cov txheej txheem ntawm cov protein, uas tuaj yeem sib cais tau raws li cov protein ntau ntawm cov protein.
Enzyme sib cais: raws li kev sib cais, kev sib cais thiab huv ntawm cov enzymes yog qhov tseem ceeb rau kev kawm ntawm lawv cov qauv thiab ua haujlwm. Gel cov kab ntawv pom kev muab cov txheej txheem sib cais thiab ua tau zoo.
Molecular Biology
Nucleic acid sib cais: txawm hais tias kev sib cais ntawm cov nucleic acids yog ntau dua li cov txheej txheem chromatography, Gel hloov cov tshuaj chromatography, Gel Hloov Cov Hluav Taws Xob
Polysaccharid Kev Tsom Xam: Ua ib chav kawm tseem ceeb ntawm cov macromolecules, cov molecular hnyav faib thiab muaj pes tsawg leeg ntawm polysaccharivides yog qhov tseem ceeb rau kev nkag siab lawv cov haujlwm. Gel Cov Lus Pom Zoo muab cov qauv nrawm thiab muaj tseeb.
Kev Tshawb Fawb Yeeb Tshuaj thiab Kev Loj Hlob
Kev Ntsuam Xyuas Yeeb Tshuaj: Hauv kev txhim kho kev tsim tshuaj, nkag siab cov kev sib xyaw ntawm cov tshuaj thiab nws cov roj ntsha hnyav yog qhov tseem ceeb rau kev tswj cov tshuaj zoo. Gel Tsis teb daim ntawv tuaj yeem muab txoj kev ua haujlwm zoo.
Cov tshuaj ntxuav yeeb tshuaj: Los ntawm cov khoom xyaw ua kom haum, cov khoom xyaw nquag hauv cov tshuaj tuaj yeem ua kom huv thiab cov kev ua yeeb yam ntawm cov tshuaj tuaj yeem hloov kho.
Lwm daim teb
Saib xyuas ib puag ncig: Gel Kev Pom Cov pa roj hmab kuj tseem siv tau rau kev sib cais hauv cov dej hauv dej, cov pa phem organic, thiab lwm yam.
Khoom Noj Khoom Haus Kawm Tshawb Fawb: Hauv Cheeb Tsam Kev Tshawb Fawb Khoom Noj Khoom Haus, Gel Cov Khoom Noj Khoom Haus Rau Kev Sib Txawv thiab Kev Ntsuam Xyuas ntawm cov as-ham, ntxiv, thiab lwm yam, hauv cov zaub mov.
Hauv cov ntsiab lus xaus, raws li dav siv kev sib cais tshuab, lub luag haujlwm Gel ua lub luag haujlwm tseem ceeb hauv ntau cov teb xws li Biochemistry, Molecular Biology, tshuaj tshawb fawb thiab kev loj hlob. Nws cov kev faib tawm feem ntau yog raws li cov kuab tshuaj pleev cov gels, thiab cov tau txais kev thov siv ntau yam los ntawm kev tshawb fawb yooj yim rau kev lag luam tsim khoom. Nrog kev txhim kho kev txuas ntxiv ntawm cov thev naus laus zis, kev thov kev cia siab ntawm Gel Portfation kem yuav muaj dav ntau.
Qhov zoo thiab qhov tsis zoo
Yam uas pab
Siv tau sib cais muaj peev xwm sib cais
Cov kua tsib ua kom raws cai tuaj yeem sib cais ntawm cov tshuaj sib txawv ntawm cov khoom hnyav molecular los ntawm kev siv lub pel loj ntawm cov gel raws li kev sib cais. Txoj kev sib cais no yog ua raws li cov khoom siv lub cev thiab tsis cuam tshuam nrog cov tshuaj lom neeg siv tshuaj, yog li nws yog xaiv tau heev thiab muaj tseeb. Nyob rau tib lub sijhawm, vim muaj nyob ntawm ib tug ntau ntawm cov qauv micropore hauv cov roj av, kev sib cais rau cov micrustruses rau diffusion thiab nrawm, yog li txhim kho kev sib cais.
Lub sijhawm nrawm
Piv nrog rau lwm cov chromatographic sib cais txoj kev, lub sij hawm ncov ntawm Gel Fictration kem yog feem ntau luv dua. Qhov no tsuas yog vim tias kev sib cais ntawm cov txheej txheem Gel ntawm Gelecular loj thiab tsis tas yuav tsum muaj cov tshuaj tiv thaiv ntau yam ua lwm txoj hauv kev. Raws li qhov tshwm sim, cov qauv txav sai dua hauv cov khoom noj khoom noj khoom haus hauv Gel thiab tuaj yeem sib cais hauv lub sijhawm luv.
Zoo biocompatibility
Cov gel cov ntaub ntawv siv hauv cov kua txiv kab ntawv yog feem ntau yog biocompomple thiab tsis ua kev puas tsuaj los yog degrade bioaction tshuaj. Qhov no ua rau cov Gel tsis tau zoo rau hauv kev sib cais ntawm cov tshuaj lom neeg xws li, thiab lwm lub sijhawm, thiab lwm yam.
Siab Tshaj Tawm Rov Qab
Cov Gel Cov Lus Cog Tseg rau sab tsis yog khaws cov impurities lossis cov seem ntawm nplooj ntawv thaum lub sijhawm sib cais, yog li nws muaj qhov kev ua haujlwm siab. Qhov no txhais tau tias thaum lub sijhawm sib cais, cov hom phiaj hom yuav tau txais tau zoo los, thaum lub impurities tuaj yeem raug tshem tawm zoo. Qhov no yog qhov tseem ceeb rau cov chaw uas xav tau cov tshuaj purity muaj zog siab, xws li kev tshawb fawb tshuaj yeeb thiab kev tsim khoom, thiab lwm yam.
Ntau yam ntawm cov ntawv thov
Gel Cov ntaub ntawv lim dej muaj ntau cov ntawv thov thiab tuaj yeem siv rau ntau yam sib txawv ntawm cov qauv sib cais thiab tsom xam. Seb nws yog cov macromolecules xws li cov protein, enzymes, thiab lwm yam, lossis lwm yam, tuaj yeem sib cais thiab tshuaj xyuas los ntawm cov kua zaub kab. Qhov no ua rau cov gel tso cai rau kab lus ib qho yooj yim thiab ntau yam txheej txheem sib cais.
Yooj yim rau kev khiav lag luam thiab tswj hwm
Kev ua haujlwm ntawm cov pa xel kab yog qhov yooj yim thiab tsis tas yuav tsum muaj cov cuab yeej ua haujlwm thiab kev ua haujlwm. Nyob rau tib lub sijhawm, vim hais tias cov khoom lag luam gel nws tus kheej muaj qhov ruaj khov thiab ruaj khov, tus saib xyuas tus nqi ntawm lub gel Daim ntawv qhia yog tseem tsawg. Qhov no ua rau cov gel pel Daim ntawv qhia txog gel ib qho yooj yim sib cais thev naus laus zis los ua cov muaj npe nrov thiab ua ntawv thov.
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Tsis yooj yim
Txawm hais tias Gel cov lus pom kev muaj peev xwm sib cais ua tau zoo, lawv cov kev daws teeb meem yog tsawg. Qhov no feem ntau yog vim tias kev sib cais ntawm cov txheej txheem gel ntawm cov gel qhov sib txawv, thiab rau cov tshuaj nrog cov hnyav, cov nyhuv sib cais tsis pom tseeb. Yog li ntawd, nyob rau hauv cov cheeb tsam uas yuav tsum muaj kev sib cais siab, xws li cov kev tshawb fawb txog proteomics, cov khoom noj khoom haus uas pom kev zoo tshaj plaws.
Lub peev xwm Peak ntawm Gel Daim Ntawv Cog Lus rau sab yog txwv, uas yog, tus naj npawb ntawm cov qauv uas tuaj yeem muab tau nyob hauv ib qho kev sib cais yog txwv. Qhov no feem ntau yog vim tias micropore cov qauv nyob hauv cov gel hais, thiab thaum cov qauv ntim dhau ntawd, nws yuav ua rau muaj teeb meem xws li cov qauv micropore lossis cov qauv tsis txaus. Yog li ntawd, hauv cov cheeb tsam uas cov qauv loj yuav tsum tau ua, xws li kev lag luam pentration, gel chivtion pentration nrog lwm cov kev sib cais.
Cov Gel Cov Lus Cog Tseg Daim Ntawv muaj cov qauv siab ntawm cov qauv, uas yuav tsum tau tus qauv kom muaj solubility zoo thiab ruaj khov nyob rau theem mobile. Nyob rau tib lub sijhawm, cov khoom siv molecular ntau ntau yam ntawm cov qauv tseem yuav tsum tau muaj mob kom tsis txhob muaj kev sib cais tsis zoo los yog hnyav me me. Tsis tas li ntawd, rau qee hom kev kuaj tshwj xeeb uas muaj cov khoom siv tsis zoo lossis cov tshuaj uas nyuaj rau yaj, yuav tsum muaj ua ntej kev sib cais lossis lwm txoj kev sib cais.
Tus nqi ntawm daim ntawv foob pob hluav taws xob yog tus nqi siab, feem ntau vim tias tus nqi siab ntawm cov khoom lag luam gel thiab cov peev txheej kev ua lag luam rau cov txheej txheem npaj. Yog li ntawd, hauv cov cheeb tsam uas yuav tsum tau hloov chaw ntau zaus, xws li kev tshawb tshuaj yeeb thiab kev txhim kho, cov nqi kev pab thiab cov nqi yuav nce ntxiv.
Cov txheej txheem kev sim ntawm Gel cov lus pom kev yog lub sijhawm sib cais, feem ntau vim tias kev sib cais yuav siv sij hawm ua kom tiav. Nyob rau tib lub sijhawm, hauv kev sib cais cov txheej txheem tseem yuav tsum tau ua cov qauv tshwm sim, seem sib npaug thiab cov kauj ruam kom tau txais txiaj ntsig, cov kauj ruam no yuav tsum tau siv qee lub sijhawm ua tiav. Yog li ntawd, nyob rau hauv cov cheeb tsam uas yuav tsum muaj kev sib cais ceev, xws li kev soj ntsuam ib puag ncig, thiab cov khoom noj zoo tshaj plaws.
Cim npe nrov: Gel Cov Lus Pom Cov Tshuaj Chromatography Sab, Tuam Tshoj Gel Portration Cov Tshuaj Quav Stromatography Sail Manufacturers, lwm tus neeg, Hoobkas
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