Classical Column Chromatography
Classical Column Chromatography
Classical Column Chromatography
Classical Column Chromatography
Classical Column Chromatography

Kem classaphy

Name: Tuam Tshoj cov chav tsev chromatographyratographyraphyraphyraphyRers Cov Khoom Siv Hoobkas - Mekas Service
Brand: Ua tiav
SKU: 262494904

1.} iav chromatographic kem
{{0 {}} chromatographic kem (hloov hom)
{{0 {}} chromatographic kem (phau ntawv)
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais

Xa kev nug

Tsham tam sim no

Hauj lwm lawm

Kev tsis

Kem classaphyPuas yog cov txheej txheem sib cais dav dav dav dav siv hauv kev ua kom huv thiab sib cais ntawm cov tshuaj sib xyaw ntawm cov khoom sib xyaw ntawm ib ntu thiab cov theem mob uas ntws los ntawm nws.

Cov theem ruaj khov, feem ntau yog cov khoom ruaj khov zoo li Silica theem-}} Cov tshuaj sib xyaw ua ke nrog cov hmoov sib txawv nrog cov theem nyob ruaj khov nrog cov theem ruaj khov raws qib ntawm lawv cov tshuaj lom neeg, xws li polarity, loj, thiab affinity rau adsorbent {}}

Cov tebchaw nrog kev sib txuas lus muaj zog nrog theem nyob ruaj khov mus rau hauv kev sib cais los ntawm kev sib xyaw kom nrawm dua rau hauv cov kev sib xyaw kom nrawm dua rau tus kheej .

Nws yog qhov muaj nuj nqis rau nws yooj yim, versatility, thiab tus nqi-teev cais, kom tau ntau yam kev sib cais vim tias nws cov khoom siv tau thiab ua kom muaj txiaj ntsig zoo rau ntau yam ntawm cov kev siv .

Tsis

Column chromatography parameter | Shaanxi Achieve chem-tech

Cov ntsiab cai yooj yim

Classical column chromatography | Shaanxi Achieve chem-tech

Kem classaphy, tseem hu ua kua-khov chratography, yog ib txheej txheem rau kev sib cais yog cov sib xyaw ua ke yog cov khoom sib cais tau sib cais txuas ntxiv rau hauv cov khoom sib cais txuas ntxiv los ntawm kev txiav tawm txawv Kev faib cov coefficients ntawm theem chanase thiab mobile theem.

Nyob rau hauv kem chromatography, lub chaw nyob ruaj khov feem ntau yog muaj ntawm cov khoom siv hydrogen, thiab tuaj yeem ua rau cov pa roj av thiab lwm yam kev sib cuam tshuam, ua rau muaj kev sib cuam tshuam nrog kev ua haujlwm. rau kev tshem tawm ntawm solute molecules los ntawm cov theem chaw ruaj khov, thiab nws xaiv yuav tsum raug txiav txim siab raws li kev sib cav thiab cov adsorption ntawm adsorbent {}}

Nyob rau hauv kev sib cais cov txheej txheem, cov sib tov tov yog qhia rau hauv nplooj ntawv nrawm nrawm, yog li lawv tau maj mam li cas txawm li cas los xij, cov khoom siv nrog tsis muaj zog polarity tau yooj yim nqa mus los ntawm cov mobile theem, thiab cov kev txav nrawm nrawm nrawm rau ntawm kab ntawv kom ua tiav kev sib cais {}}

Amino Acid Kev Ntsuam Xyuas

1. Lub ntsiab lus

Amino acid tsom xam feem ntau yog ua siv kua chromatography, ntawm uaskem classaphyyog cov txheej txheem feem ntau . Nyob rau hauv hom kev sib cais ua ntej cov qauv sib zog tuaj yeem hloov kho cov khoom siv hluav taws xob ntxiv rau cov amino acids rau chromatographic kem .

2. Kauj Ruam

Cov Qauv Npaj Ua Qauv: Cov tshuaj tiv thaiv muaj protein yog cov amino acids {}} Qhov no yuav ua tiav kev kho mob hauv hydrolysis, xws li tshem tawm cov protein ntau {}}
Derivatization: Txhawm rau txhim kho qhov rhiab ntawm amino acceting excection thiab post}}}}}}}}}}} {), metyl chlorofluorene (FMOC-CI), Phenyl Isothiocyanate (Stanc), {} dinitroflupuzene (FDNB), thiab lwm yam.
Xaiv Rau Cim: Xaiv lub kab haum rau kem haum rau kev tsom xam, xws li ion Txauv kem, C18 kem, C8 .
Chromatographic Chaw: Teeb tsim qauv Chromatographic tsim nyog suav nrog cov phag mobile, xws li tsim cov buffers.
Cov qauv tshuaj xyuas: tus qauv tom qab kev txiav nyiaj rau hauv tus qauv checkography, cov ntsiab lus ntawm cov amino acids nyob rau hauv cov qauv ntawm cov qauv tuaj yeem yog qhov ntsuas tau ntau.
Cov ntaub ntawv ua: Raws li cov ntaub ntawv siab tshaj plaws thiab cov ntaub ntawv nkag tau los ntawm kev tsom xam cov qauv, thiab cov ntaub ntawv yog ua tiav thiab cov txiaj ntsig yog qhia .

3. Cov piv txwv thov

Muaj ntau yam ntawv thov ua piv txwv hauv cov amino acid tsom xam, thiab muaj ntau kis tshwj xeeb muaj nyob hauv qab no:

Kev kuaj pom ntawm cov amino acids tseem ceeb: kev siv tuaj yeem tshawb xyuas cov ntsiab lus tseem ceeb, Lucine, Lucy {}} Cov pa roj hmab tseem ceeb thiab kev noj qab haus huv.
Kev txiav txim siab ntawm amino acids nyob rau hauv Suav tshuaj: hauv kev tsom xam cov ntsiab lus ntawm kev ua haujlwm ntawm Suav teb hauv Wuji Bauring tuaj yeem siv rau kev sib cais thiab kev txiav txim siab ntawm lwm yam amino acids .
Kev txiav txim siab ntawm amino acids nyob rau hauv cov zaub mov: amino acid cov ntsiab lus hauv cov zaub mov thiab txiav txim siab siv cov khoom noj khoom haus tsis zoo thiab txiav txim siab} tib lub sijhawm, tus qauv kuj tuaj yeem yog siv los txiav txim siab cov ntsiab lus ntawm cov amino acids nyob rau hauv lwm cov zaub mov, uas muab cov khoom tseem ceeb rau cov kev tshuaj xyuas khoom noj zoo thiab zaub mov zoo {}}

4. Cov kev ceev faj

Nyob rau hauv amino acid tsom xam, nws yog ib qho tsim nyog los xaiv cov tshuaj tiv thaiv derivatization uas tsim nyog los txhim kho qhov rhiab heev thiab kev tshawb nrhiav .
Qhov kev xaiv ntawm kem thiab kev teeb tsa ntawm chromatographic mob muaj qhov cuam tshuam tseem ceeb rau cov qauv ua haujlwm thiab kev xav tau .
Nyob rau hauv cov txheej txheem kev tsom xam, nws yog ib qho tsim nyog los them sai sai rau kev npaj thiab kev cia ntawm cov qauv kev paug thiab kev txwv tsis pub muaj .

Dab tsi yog derivive kev kho mob

Derivatization yog ib qho tseem ceeb qauv ua ntej cov txheej txheem hauvkem classaphy, uas lub hom phiaj los txhim kho kev kawm ua tau zoo thiab kev sib cais ntawm analytes {}}}}} Cov lus qhia ntxaws ntxaws ntawm cov txheej txheem derivatization:

► Lub hom phiaj ntawm kev kho mob derivative
Lub hom phiaj tseem ceeb ntawm derivatization yog hloov lub cuab tam li ntawd lawv tsim nyog rau kev sib cais thiab kev ua tiav tuaj yeem ua tiav cov hom phiaj hauv qab no:

1) Kev Tshawb Nrhiav Kev Kawm Tau Zoo: Los ntawm kev qhia cov pab pawg ua haujlwm tau yooj yim ntawm cov teeb liab, txhim kho qhov kev paub zoo thiab qhov tseeb ntawm kev tshawb pom.
2) Hloov cov qauv molecular lossis polarity: Kho cov polarity ntawm kev ntsuas ntawm tus tshuaj ntsuam ntawm qhov kev sib cais ntawm kem thiab sib cais .
3) Kev siv hluav taws xob nce ntxiv: rau qee qhov tsis yog verivatales tuaj yeem txhim kho lawv cov volatility tuaj yeem txhim kho lawv txoj kev qhib volatility tuaj yeem cais thiab tshawb xyuas hauv cov roj chromatographyrography .
4) Kev ruaj khov: Cov pab pawg ua haujlwm ruaj khov hauv kev tiv thaiv tuaj yeem tiv thaiv kev ua kom tsis txhob muaj kev cuam tshuam lossis hloov pauv thaum sib cais thiab nrhiav kom tau.

Classical column chromatography | Shaanxi Achieve chem-tech

► Hom kev kho mob derivative
Derivatization feem ntau muab faib ua ob hom: pre-kem derivatization thiab post-kem derivatization:

1) pre-kem derivation:
Ua ntej tus kws tshuaj ntsuam nkag rau hauv kem, nws siv tshuaj yooj yim nrog cov neeg ua tus nqi los ua cov derivates .
Cov derivatives yog tom qab sib cais thiab kuaj pom ntawm ib kem .
Preplumn derivatization muaj qhov zoo ntawm cov tshuaj tiv thaiv dawb thiab yooj yim heev, tab sis nws tseem yuav qhia cov tshuaj impurities lossis plam.
2) Ncej kem derivation:
Cov kws tshuaj ntsuam tau sib cais thawj zaug ntawm cov chromatographic kem thiab tom qab cheev xwm txheej nrog cov derivants hauv cell cell .
Lub txiaj ntsig derivatives yog ces nkag mus rau hauv lub ntes mus nrhiav kom tau paub .

Cov khoom siv tom qab tau muaj qhov zoo ntawm qhov zoo tshaj plaws reprucibility thiab tsawg dua cov twj siv thiab khoom siv ntxiv.
Kev thov cov piv txwv ntawm derivative ua
Nyob rau hauv extraction thiab sib cais ntawm cov khoom siv tshuaj, nws ua ke nrog kev siv cov tshuab derivative ua tau tuaj yeem ua tiav cov ntawv thov hauv qab no:

1) Kev Tshawb Xyuas Kev Tshawb Xyuas: Piv txwv, kev soj ntsuam ntawm cov amino acids yooj yim tuaj yeem nce cov teeb meem yooj yim tuaj yeem ua rau pom kev zoo siab ntawm kev tshawb pom.
2) Txhim kho cov nyhuv sib cais: rau qee cov tebchaw zoo sib xws, yog li kho lawv cov polaritional, yog li kho lawv cov polaritional, yog li kho lawv cov polaritional, yog li kho lawv cov polaritional thiab txhim kho cov nyhuv sib cais.
3) Kev tiv thaiv ntawm pawg ua haujlwm raug: Rau cov kws tshuaj ntsuam muaj cov pab pawg ua haujlwm tsis zoo, hauv cov txiaj ntsig carbothydration, hauv}.}.. sib cais .

Classical column chromatography | Shaanxi Achieve chem-tech

Kem classaphy, cov txheej txheem ua tiav hauv kev sib cais nrog kev sib cais nrog kev nce qib hauv cov ntaub ntawv thiab kev siv hauv ob qho kev lag luam .

Ib qho kev txhawb nqa yog kev sib koom ua ke ntawm kev ua haujlwm tshiab parses {}} Cov ntaub ntawv tso cai rau cov khoom siv high-{2} {{4 4}}

Tsis tas li ntawd, nws yog nce tau ua ke nrog kev paub txog cov txheej txheem ntau ntxiv, txhawm rau txhim kho cov txheej txheem sib xyaw ua ke, txhawm rau txhim kho cov txheej txheem zoo li cov metabolomics, txhawm rau txhim kho cov txheej txheem zoo li cov metabolomics, txhawm rau txhim kho cov txheej txheem zoo li metabolomics, txhawm rau txhim kho cov txheej txheem zoo li metabolomics, los txhim kho cov txheej txheem zoo li cov metabolomics, txhawm rau txhim kho cov txheej txheem zoo li metabolomics, txhawm rau txhim kho cov kev siv hluav taws xob zoo li cov metabolomics, txhawm rau txhim kho cov txheej txheem zoo li cov metabolomics, txhawm rau txhim kho cov kev siv hluav taws xob zoo li cov metabolomics, txhawm rau txhim kho cov txheej txheem zoo li metabolomics thiab proteomics .

Hauv kev xaus, cov lus dag yav tom ntej nyob rau hauv nws kev yoog rau cov ntaub ntawv tshiab, kev sib koom ua ke nrog kev ua lag luam niaj hnub niaj hnub no.

Cim npe nrov: kem classaphy chromatography, Tuam Tshoj Classical kem chromatograph manufacturers, lwm tus neeg, Hoobkas

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