Beaker conical flask
1) nqaim-lub qhov ncauj fwj: 50ml ~ 10000ml;
2) be fwj loj: 50ml ~ 3000ml;
3) Horn qhov ncauj: 50ml ~ 5000ml;
4) Lub qhov ncauj dav dav: 50ml / 100ml / 250ml / 500ml / 1000mL;
5) Conical flask nrog npog: 50ml ~ 1000mL;
6) Ntsia Hlau Congical Flask:
a. Lub hau dub (cov teeb tsa dav dav): 50ml ~ 1000mL
b. Txiv kab ntxwv hau (tuab hom): 250ml ~ 5000ml;
2. Ib leeg thiab ntau lub qhov ncauj puag ncig lub hauv qab flask:
1) Tib qho lub qhov ncauj puag ncig cov flask: 50ml ~ 10000ml;
2) Inclined peb-lub qhov ncauj flask: 100ml ~ 10000ml;
3) Inclined plaub-qhov ncauj flask: 250ml ~ 20000ml;
4) ncaj peb-lub qhov ncauj flask: 100ml ~ 10000ml;
5) Ncaj plaub-qhov ncauj flask: 250ml ~ 10000ml.
*** Daim ntawv teev npe rau tag nrho saum toj no, nug peb kom tau txais
Hauj lwm lawm
Kev tsis
A beaker conical flask, Hloov npe hu ua ErlenMeyer flask (tom qab nws tus tsim, ErlenMeyer), yog hom ntawm cov kab hlau uas nws txoj kev nqaim thiab dav, conical puag. Cov qauv tsim kom ntseeg tau tias cov flask tuaj yeem tso tau ruaj khov rau ntawm chav kuaj xyuas tsis muaj tipping thiab kuj tau yooj yim sib xyaw ntawm nws cov ncauj lus.
Conical flasks yog tsim los ntawm cov ntaub ntawv xws li iav lossis yas thiab tuaj nyob rau ntau qhov ntau thiab tsawg thiab muaj peev xwm. Lawv tau siv dav hauv Chemistry thiab Biochemistry lub chaw kuaj mob rau ntau lub hom phiaj, suav nrog kev tuav thiab sib xyaw cov tshuaj reactants, ua cov khoom siv distillation.
Ib qho beaker, kuj nquag hu ua lub khob loj-qhov ncauj, yog ib qho khoom siv hauv khw feem ntau siv rau kev sib xyaw, cua sov, thiab khaws cia tshuaj yeeb. Nws feem ntau NTIM qhov qhib uas ua kom yooj yim nchuav thiab nplawm cov ntsiab lus. Ua los ntawm cov ntaub ntawv xws li iav lossis yas, beakers muaj nyob rau ntau qhov ntau thiab tsawg thiab muaj peev xwm siv tau sib txawv.
Vim nws qhov ncauj dav thiab kev tsim qauv ruaj khov, ib lub beaker yog qhov zoo tagnrho rau cov kev siv hauv Chemistry, Biology, thiab lwm thaj chaw science. Piv txwv li, nws tuaj yeem siv los ntsuas thiab sib xyaw cov tshuaj sib xyaw, cov kev daws teeb meem cua sov, lossis txawm tias yog lub thawv rau tuav cov qauv thaum sim.
Cov lus qhia
Cov ntawv thov hauv Biology
Hauv DNA cov kev sim extraction extraction, ib tug voosbeaker (conical flask)Feem ntau siv los sib tov, do, thiab kub npaum li cas ntawm cov kua ua piv txwv. Hauv qab no yog cov txheej txheem ib qib zuj zus rau kev sim siv lub voos voos kauj ruam rau DNA extraction, siv cov khoom cog ua piv txwv:
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Lub hom phiaj ntawm kev sim: Cov khoom siv sim: |
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Kev sim ua kauj ruam:
1. Qauv Npaj:
Zom kwv yees li 10 g ntawm tshiab los yog khov tsob nroj nplooj kuaj mus rau hauv cov hmoov zoo uas siv tshuaj khib thiab pestle hauv cov kua nitrogen.
2. Qauv Hloov:
Hloov cov av zoo hmoov rau cov dej khov ua ntej txias 500 ml xob laim los 500 ml ntawm nib ntas thiab sib tov nrawm.
3. Kev maj sib xyaw:
Cov ntsiab lus ntawm lub voos xwm txheej voos tau maj mam muab do rau dej khov rau 10 feeb ntawm 100 x rpm.
4. Pom:
Cov dej sib tov tau lim los ntawm ob txheej ntawm daim ntaub pua chaw thiab ob txheej ntawm miracloth thiab qhov kev ncua kev kawm nuclear tau sau tau siv lub funnel.
5. Sau ntawm filtrate:
Qhov freatrate tau faib rau hauv ob 50- ml daim duab peb sab thiab centrifuged ntawm 2400 × g rau 12 min ntawm 4 degree; tus supernatant tau muab pov tseg.
6. Ntxuav:
Siv tus txhuam me me, rov ua dua cov nag lossis daus nyob hauv txhua daim duab cua sov nrog 20 ml ntawm cov dej khov nab kuab, sib txuas ua ke, thiab centrifuge ntxiv.
7. Rov ua dua li ntxuav:
Rov ua dua cov kauj ruam 5 thiab 6 kom txog thaum cov xim ntsuab ploj mus thiab qhov kev tshem tawm tau meej.
8. DNA Nag ntuj:
Discard the supernatant and add 0.5-2 mL of ice-cold NIB buffer to each triangular beaker and gently suspend the precipitate.
9. Txuas ntxiv ntawm Ctab Tsis yog:
Ncuav 20 ml ntawm 2 × ctom (65 degree) tsis yog, sib xyaw tam sim ntawd, thiab ua kom tiav ntawm 65 degree rau chav sov.
10. Chloroform extraction:
Sib tov nrog cov khoom ntim sib npaug ntawm chloroform los ntawm maj mam tsaj lossis inverting thiab centrifuge thiab centrifuge ntawm chav sov.
Hloov Supernatant:
Hloov 20 ml ntawm supernatant rau lub raj tshiab, ntxiv 2% ctab tsis, sib tov maj mam muab thiab ua kom tiav ntawm 65 degree.
Extract dua nrog chloroform:
Extract dua nrog chloroform thiab centrifuge ntawm chav sov.
DNA nag los:
Hloov 15 ml ntawm supernatant rau lub xyoob ntoo tshiab, ntxiv ib qho sib npaug ntawm 1 × ctom kev sib xyaw ua ntej los ntawm inversion to precipite genomic DNA.
DNA Resuspension:
Tom qab centrifugation, supernatant tau muab pov tseg thiab cov DNA precipitates tau raug tshem tawm nrog 600 μl ntawm no te cov ntsev ntau.
DNA ntxuav:
Hloov qhov raug tshem tawm rau {2- ml tube, ntxiv 1.2 ml ntawm ethanol, sib tov maj mam kom txog thaum lub chav sov, thiab tawm ntawm chav tsev kub rau 5 feeb.
DNA PURIFATION:
Ua cov kauj ruam ntxiv ua kom huv ntau xws li Erasol nag lossis resuspension li xav tau.
Cov kev ceev faj:
Nco ntsoov siv freshly ua tsis tiav rau megabase-loj DNA kev rho tawm.
Zam kev ua kom lub cev nyhav ntau dhau thaum ua cov hnoos qeev kom tswj hwm DN kev ncaj ncees.
Tswj cov mob tsis muaj mob thaum lub sij hawm sim los txo DNA degradation.
Nrog rau qhov kev sim no, zoo siab, siab molecular hnyav golecule gutracted rau ib hnub, uas yog qhov tseem ceeb tshaj plaws piv rau lub sijhawm ib txwm muaj rau ib-lub lim tiam sijhawm.
Lawv tau siv hauv Chemistry, Biology, thiab Microbiology Protatories rau cua sov, sib tov, thiab kev hloov cov tshuaj lossis cov tshuaj tiv thaiv. Kev siv tshwj xeeb suav nrog cov kev sim siab tshaj plaws, cov dej npau npau, thiab DNA kev sib cav sib ceg.
Tuav ntawm Xwm Txheej Ceev
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Thaum sivbeaker conical flaskTxhawm rau ua cov kev sim, thaum muaj xwm txheej ceev, tiv thaiv kev ntsuas tus kheej kom paub tseeb, thiab tiv thaiv cov cuab yeej sim thiab reagents ntau li ntau tau. Nov yog qee cov lus teb tshwj xeeb:
Hluav Taws Kub Xwm Ceev
Txiav tawm qhov hluav taws kub tam sim ntawd:
Yog tias muaj hluav taws nyob hauv lossis ze raubeaker conical flask, qhov hluav taws xob hluav taws yuav tsum tau txiav tawm tam sim ntawd, xws li kaw cov roj av roj av lossis tshem lub hwj chim ntsaws.
Siv Cov Hluav Taws Xob Hluav Taws Xob:
Xaiv cov tua hluav taws uas tsim nyog raws li hom hluav taws. Rau cov hluav taws ua kua roj, xws li dej cawv, Etaws, thiab lwm yam, cov hmoov ntoo qhuav hluav taws xob tuaj yeem siv; Rau cov tshuab hluav taws xob hluav taws xob, lub zog yuav tsum tau txiav tawm ua ntej, thiab tom qab ntawd siv cov pa roj carbon, tua hluav taws tua hluav taws.
Kev Tawm Tawm thiab Tswb:
Yog tias cov hluav taws tsis tuaj yeem tswj tau, cov neeg ua haujlwm kuaj cov neeg ua haujlwm yuav tsum tau khiav tawm hauv lub xov tooj tam sim ntawd thiab lub xov tooj hluav taws xob tsis raug hu ua.
Ua tib zoo rau tus kheej tiv thaiv:
Thaum tua hluav taws, cov neeg sim yuav tsum muaj cov khoom siv tiv thaiv, xws li cov khaub ncaws hnav tiv thaiv, ua pa apparatus, thiab lwm yam.
Kev tuav ntawm kev tawg ceev ceev
Kev Tawm Tsam Kev Tawm Tsam
Yog tias muaj kev tawg, tus sim yuav tsum khiav tawm ntawm qhov chaw kom tsis txhob raug mob ob.
Txiav tawm lub zog thiab cua
Cov ntaub ntawv ntawm kev ua kom muaj kev nyab xeeb, txiav ceev ceev ntawm lub zog thiab cua xa mus rau hauv chav kuaj kom tiv thaiv kom tsis txhob muaj qhov xwm txheej ntxiv.
Thawj pab thiab tswb
Muab thawj zaug pabcuam rau kev raug mob, xws li hemostasis, bandging, thiab lwm yam, thiab ua kev hu thaum muaj xwm ceev. Nyob rau tib lub sijhawm, qhia qhov xwm txheej rau tus neeg kuaj xyuas chav kuaj thiab chav tiv thaiv kev nyab xeeb.
Tiv thaiv lub xaib
Tiv thaiv qhov chaw sib tsoo rau kev tshawb nrhiav txuas ntxiv thiab tshuaj ntsuam xyuas thaum ua kom muaj kev nyab xeeb.
Tshuaj Yeeb Nkab Reagent Duav Kho Mob
Tam sim ntawd quarantine
Yog tias cov tshuaj lom neeg tshuaj lom neeg hauv lub conical flask txeej, thaj chaw xau dej yuav tsum raug cais tawm tam sim ntawd los tiv thaiv kev kis tus reagent.
01
Kev tiv thaiv tus kheej
Cov neeg ua haujlwm sim yuav tsum cov khoom siv tiv thaiv uas tsim nyog, xws li cov khaub ncaws tiv thaiv uas tsim nyog, hnab looj tes kom tsis txhob sib chwv nrog kev sib chwv.
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Sau thiab nruab nrab
Siv cov cuab yeej sau uas tsim nyog (xws li xuab zeb, roj nqus paj rwb, thiab lwm yam) kom sau cov khoom siv reagent thiab neutralizing nws nrog cov khoom ntawm lub reagent.
03
Kev ntxuav thiab dim pa
Ntxuav cov chaw xau dej kom huv si thiab siv cov khoom siv cua kom tshem tawm cov pa roj teeb.
04
Kev ceev faj kev nyab xeeb kev nyab xeeb
Paub txog kev kuaj xyuas cov txheej txheem kev nyab xeeb
Cov neeg saib xyuas kev nyab xeeb yuav tsum paub txog cov txheej txheem kev nyab xeeb thiab kev ua kom xwm ceev kev ntsuas kom lawv tuaj yeem teb tau sai sai thaum muaj xwm txheej ceev.
Kev tshuaj xyuas ib txwm muaj cov khoom siv
Kuaj cov khoom siv tsis tu ncua kom ntseeg tau tias nws yog qhov zoo kom tsis txhob muaj kev sib tsoo los ntawm cov cuab yeej tsis ua haujlwm.
Khaws lub lab ntxuav
Khaws lub sim kom huv thiab kev txiav txim siab kom tsis txhob muaj cov khib nyiab cuam tshuam nrog kev sim lossis kev phom sij kev nyab xeeb.
Kev Pab Cuam Tshuam Kev Nyab Xeeb
Kev ua kev nyab xeeb kev nyab xeeb rau kev kawm kev nyab xeeb rau cov neeg ua haujlwm uas sim ua kom lawv muaj peev xwm tuav kev kub ntxhov.
Sau cov kev faj sau cia
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Thaum ua kev sim tshuaj nrog beaker conical fwj, cov ntaubntawv yog tus yuam sij los saib xyuas qhov tseeb, kev tshawb fawb thiab kev tshawb fawb ntawm cov ntaub ntawv sim. Cov ncauj lus kom ntxaws, raug thiab muaj kev npaj cov ntaub ntawv muaj peev xwm tsis tuaj yeem muab lub hauv paus kev sim ua kom pom tseeb, tab sis kuj tseem sau cov ntawv nyeem txuas ntxiv, ntawv sib txuas lus thiab kev sib txuas lus kev tshawb fawb. Ntawm no yog qee cov ncauj lus kom ntxaws ceeb toom kom nco ntsoov thaum sim nrog beaker conical fwj:
Cov ntsiab cai ntawm cov ntaubntawv povthawj siv
Cov ntaub ntawv yuav tsum ua raws li kev ntsuas qhov tseeb uas tau sim ua tiav thiab cov ntaub ntawv, tsis txhob muaj kev xav lossis kev tsis ncaj ncees. Txhua cov ntaub ntawv yuav tsum tau piav qhia nyob rau hauv cov hom phiaj thiab hom lus thiab tshwj xeeb.
Cov ntaub ntawv kaw tseg yuav tsum muaj tseeb li sai tau, suav nrog cov nuj nqis, chav nyob, ntsuas kev ntsuas lossis kev kwv yees, qhov tsis paub tseeb yuav tsum tau cim kom pom tseeb.
Cov ntaub ntawv yuav tsum npog tag nrho cov txheej txheem ntawm kev sim, los ntawm kev npaj theem mus rau thaum kawg ntawm kev sim, suav nrog kev ntsuas noj, ntsuas cov txheej txheem ua haujlwm tau, thiab lwm yam.
Cov ntaub ntawv yuav tsum tau zoo thiab nkag siab yooj yim. Siv cov taub hau ntshiab, cov ntu, thiab cov npe txhawm rau npaj cov ntaub ntawv rau kev txheeb xyuas txuas ntxiv thiab tsom xam.
Cov ntaub ntawv yuav tsum muaj cov ntaub ntawv txaus kom lwm tus kws sim tuaj yeem hloov kho qhov kev sim. Qhov no suav hnub ntawm kev sim, lub npe ntawm tus sim, cov xwm txheej ntawm kev sim, tus qauv ntawm kev ntsuas, thiab lwm yam.
Npaj cov ntaub ntawv ua ntej sim
Kev sim tsim:Ua ntej kev sim ua ntej, lub hom phiaj, kev xav, kev xav tau tshwm sim, thiab kev tsim qauv yuav tsum tau sau cia kom meej. Qhov no yuav pab qhia meej cov kev taw qhia thiab lub hom phiaj ntawm kev sim.
Reagents thiab seev:Sau lub npe, purity, chaw tsim tshuaj paus, ntau tus naj npawb ntawm txhua reagents siv, nrog rau tus qauv, tshwj xeeb thiab kev ntsuas cov xwm txheej ntawm qhov ntsuas. Cov ntaub ntawv no yog qhov tseem ceeb rau kev taug kev tracavility ntawm kev sim ua.
Kev Npaj Kev Nyab Xeeb:Sau cov kev nyab xeeb muaj peev xwm kev nyab xeeb thiab kev tawm tsam hauv kev sim kom ntseeg tau tias kev nyab xeeb ntawm cov txheej txheem sim.
Cov ntaub ntawv thaum sim
Reagent ntxiv
Sau cov xaj, tus nqi, txoj kev thiab lub sijhawm ntawm txhua reagent ntxiv. Rau reagents uas yuav tsum muaj qhov raug hnyav, qhov tseeb yuav tsum tau sau cia.
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Cov txheej txheem sim
Sau cov kev sim ua haujlwm ntawm txhua kauj ruam hauv kev nthuav dav, suav nrog cov cua sov, nplawm, thiab lwm yam cov ntsiab lus tshwj xeeb thiab kev ceev faj.
02
Pom tseeb phatomena
Sau tag nrho cov xwm txheej pom thaum lub sij hawm sim, xws li kev hloov xim, kev hloov pauv ntawm qhov dej, thiab lwm yam.
03
Cov ntaub ntawv ntsuas
Sau tag nrho cov ntaub ntawv ntsuas, suav nrog qhov ntsuas kub, ntim, ntau, thiab lwm yam kev ntsuas, cov txiaj ntsig thiab qhov ntsuas nruab nrab ntawm txhua qhov ntsuas yuav tsum sau cia.
04
Tsis txawv txav
Yog tias muaj qhov xwm txheej txawv txav thaum lub sij hawm sim, xws li reagent hais, ntsuas tsis tas li ntawd, kev sim yuav tsum tau nres tam sim thiab cov txiaj ntsig yuav tsum tau sau tseg.
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Sau thiab kev soj ntsuam tom qab sim
Cov Ntaub Ntawv Kev Sib Tw
Tom qab sim, tag nrho cov ntaub ntawv kaw tseg tau sib xyaw thiab tshuaj ntsuam. Siv cov kab kos, cov ntxhuav, thiab lwm hom ntawv los visually nthuav tawm cov ntaub ntawv rau kev soj ntsuam tom ntej thiab kev sib tham.
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Kev sib tham
Raws li cov ntaub ntawv sim thiab pom tias yog tham seb puas tau sim ua kom tau txais cov kev cia siab, thiab tshuaj xyuas cov peev xwm ua tau thiab cuam tshuam. Cov txiaj ntsig uas tsis tau ua raws li kev cia siab yuav tsum muaj kev txheeb xyuas thiab sib tham.
02
Cov lus xaus thiab cov lus qhia
Raws li cov kev sim thiab tsom xam cov txiaj ntsig tau, thiab cov lus qhia yog txhim kho cov txheej txheem sim, ua kom pom kev sim lossis kev tshawb fawb ntxiv.
03
Sau Cov Ntaub Ntawv Cia
Khaws cov ntaub ntawv hais txog sim hauv chav ua haujlwm hauv qhov chaw nyab xeeb thiab nyab xeeb rau kev ntsuam xyuas txuas ntxiv thiab sib qhia. Txiav txim siab siv cov ntaub ntawv hluav taws xob lossis huab cia kom khaws cov ntaub ntawv txhawm rau txhim kho lawv cov kev nyeem tau yooj yim thiab siv tau.
04
Cov Lus Qhia Tshwj Xeeb Hauv Cov Ntaub Ntawv
Timestam
Ntxiv rau lub sijhawm ua haujlwm rau cov ntaub ntawv kom sau lub sijhawm ntawm txhua kauj ruam tseem ceeb lossis kev soj ntsuam taw tes pab soj ntsuam lub sijhawm vam khom thaum sim.
Kos Npe thiab Hnub Tim
Txhua nplooj ntawv ntawm cov ntaub ntawv yuav tsum muaj kos npe thiab hnub ntawm tus kws sim kom ntseeg tau tias muaj tseeb thiab kev tawm tswv yim ntawm cov ntaub ntawv.
Kev ceev ntiag tug
Rau cov ntaub ntawv sim uas muaj cov ntaub ntawv rhiab lossis cov ntawv sau cia, tsim nyog tsis pub lwm tus paub kom tsis txhob muaj ntaub ntawv tshaj tawm.
Kev txheeb xyuas cov ntaub ntawv hluav taws xob
Yog tias cov khoom siv hauv tshuab hluav taws xob yog siv rau sau, qhov tseeb thiab kev ntseeg tau ntawm cov khoom siv hluav taws xob yuav tsum tau kuaj xyuas kom meej tias cov ntaub ntawv tseeb.
Cim npe nrov: beaker conical flask, Tuam Tshoj beaker conical flask manufacturers, cov chaw muag khoom, Hoobkas
Tom ntej
Quartz Round Hauv Qab RirkXa kev nug
